Related Experiment Video

Updated: Mar 12, 2026

Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization
14:55

Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization

Published on: September 1, 2018

11.3K

Apoptosis induction of human endometriotic epithelial and stromal cells by noscapine

Mohammad Rasoul Khazaei1, Zahra Rashidi2, Farzaneh Chobsaz1

  • 1Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Iranian Journal of Basic Medical Sciences
|November 3, 2016
PubMed
Abstract

Insights

Noscapine effectively reduced endometriotic cell viability and increased cell death in vitro. This suggests noscapine as a potential therapeutic agent for endometriosis treatment.

Area of Science:

  • Gynecology
  • Cell Biology
  • Pharmacology

Background:

  • Endometriosis is a complex gynecologic condition with an unknown cause.
  • Noscapine, a known cancer cell suppressor, is being investigated for its potential in treating cancer-like disorders.

Purpose of the Study:

  • To investigate the apoptotic effect of noscapine on human endometriotic epithelial and stromal cells in vitro.
  • To evaluate noscapine's efficacy as a potential treatment for endometriosis.

Main Methods:

  • Endometrial biopsies from 9 endometriosis patients were used for in vitro study.
  • Cells were treated with varying concentrations of noscapine (10-100 µM) for 24, 48, and 72 hours.
  • Cell viability, nitric oxide levels, and apoptosis were assessed using colorimetric assays, Griess reagent, and AO-EB/TUNEL staining.

Main Results:

  • Noscapine significantly decreased endometrial epithelial and stromal cell viability across all tested concentrations and time points (P<0.05).
  • An increased apoptotic index was observed in cells treated with 25, 50, and 100 µM noscapine at 48 hours (P<0.05).
  • Nitric oxide concentrations did not show a significant decrease.

Conclusions:

  • Noscapine demonstrated a significant apoptotic effect on human endometriotic epithelial and stromal cells in vitro.
  • The findings suggest that noscapine could be a potential therapeutic agent for managing endometriosis.
Keywords:
ApoptosisEndometriosisNitric oxideNoscapine

More Related Videos

Two Methods for Establishing Primary Human Endometrial Stromal Cells from Hysterectomy Specimens09:15

Two Methods for Establishing Primary Human Endometrial Stromal Cells from Hysterectomy Specimens

Published on: May 23, 2014

23.8K
Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen19:44

Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen

Published on: May 30, 2012

19.3K

Related Concept Videos

Apoptosis01:30

Apoptosis

Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
16.6K
Phagocytosis of Apoptotic Cells01:17

Phagocytosis of Apoptotic Cells

Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or  immature dendritic cells. Non-professional phagocytes such as  epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes. 
Normal cells contain receptors that prevent them from being recognized...
5.4K
The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
9.2K
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
9.1K
Overview of Cell Death01:30

Overview of Cell Death

Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases.
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
10.8K