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High-performance liquid chromatography with electrospray ionization ion mobility spectrometry: Characterization, data
Martin Zühlke1, Daniel Riebe1, Toralf Beitz1
1University of Potsdam, Physical Chemistry, Potsdam.
Journal of Separation Science
|November 3, 2016
Summary
High-performance liquid chromatography coupled with electrospray ionization ion mobility spectrometry enables two-dimensional separation of complex mixtures. This advanced technique improves detection and identification of compounds, even those lacking chromophores.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Complex mixtures pose challenges for traditional separation techniques.
- Two-dimensional separation methods offer enhanced resolution and peak capacity.
Purpose of the Study:
- To develop and characterize a high-performance liquid chromatography (HPLC) system coupled with electrospray ionization ion mobility spectrometry (ESI-IMS).
- To demonstrate the system's capability for two-dimensional separation and substance identification.
Main Methods:
- Optimization of an ion mobility spectrometer for HPLC flow rates (100-1500 μL/min).
- Characterization of the 2D system using peak capacity analysis.
- Separation and detection of pesticide and amino acid mixtures.
Main Results:
- Achieved orthogonal separation in retention time and drift time dimensions.
- Resolved all 24 pesticides in a mixture, overcoming coelutions seen in 1D.
- Successfully separated 18 amino acids, including those without chromophores.
- Developed custom software based on OpenMS for automated 2D data processing.
Conclusions:
- The coupled HPLC-ESI-IMS system provides superior separation and identification capabilities for complex mixtures.
- Ion mobility spectrometry offers substance identification based on characteristic mobilities.
- The developed software facilitates efficient processing of extensive 2D data, enabling automated analysis.
Keywords:
Amino acidsElectrospray ionizationIon mobility spectrometryPesticidesTwo-dimensional separationsMore Related Videos
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