Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

FedCAD: Cross-modal semantic alignment and distillation for cross-domain heterogeneous federated learning.

Neural networks : the official journal of the International Neural Network Society·2026
Same author

Optimising POU3F4 variant interpretation through gene-specific evidence in X-linked hearing loss.

EBioMedicine·2026
Same author

Long-term restoration of auditory function in a DFNA2 mouse model by adenine base editing.

EMBO molecular medicine·2026
Same author

In vivo epigenome editing reduces circulating lipids and attenuates atherosclerosis in mice.

Journal of genetics and genomics = Yi chuan xue bao·2026
Same author

Complex peopling history and expansion events inferred from large-scale modern and ancient Y chromosome sequences.

Science advances·2026
Same author

Corrigendum to "Generation of induced pluripotent stem cell line IRDWCHi001-A from a patient with hearing loss and nystagmus carrying the heterozygous TBX2 c.977delA (p.D326Afs*42) mutation" [Stem Cell Res. 90 (2026) 103906].

Stem cell research·2026
Same journal

Two satellite repeats reveal B chromosome structural diversity in Atractylodes lancea.

Genome·2026
Same journal

A Drosophila teissieri I-element retrotransposon's ORF1p shows RNA binding cis-preference in the D. melanogaster female germline.

Genome·2026
Same journal

Abiotic stress-responsive tRNA-derived fragments in pitanga (<i>Eugenia uniflora</i> L.): regulatory roles in drought and salinity adaptation.

Genome·2026
Same journal

Diacylglycerol kinase promotes forgetting of aversive olfactory memory in Drosophila.

Genome·2026
Same journal

From Lab Bench to Public Voice: How to Incorporate Science Communication into Your Research Program.

Genome·2026
Same journal

<i>delimtools</i>: an R package for species delimitation.

Genome·2026
See all related articles

Related Experiment Video

Updated: Mar 12, 2026

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
13:14

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

9.8K

Closed-Tube Barcoding.

Nicky M Sirianni1,1, Huijun Yuan1,1, John E Rice1,1

  • 1Brandeis University, Department of Biology, Waltham, MA 02453-9110, USA.

Genome
|November 5, 2016
PubMed
Summary
This summary is machine-generated.

We developed Closed-Tube Barcoding, a new method using PCR technology to rapidly and affordably identify, catalog, and monitor global biodiversity, especially for small or rare specimens.

Keywords:
Closed-Tube BarcodingLATE-PCRLights-On/Lights-Off probesPan-phylum probesSondes noir et blanc (« Lights-On/Lights-Off probes »)codage à barres en tube fermé (« Closed-Tube Barcoding »)fluorescent signaturessignatures en fluorescencesondes pan-spécifiques

More Related Videos

BEST: Barcode Enabled Sequencing of Tetrads
12:59

BEST: Barcode Enabled Sequencing of Tetrads

Published on: May 1, 2014

10.6K
Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella
07:11

Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella

Published on: May 13, 2019

10.2K

Related Experiment Videos

Last Updated: Mar 12, 2026

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
13:14

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

9.8K
BEST: Barcode Enabled Sequencing of Tetrads
12:59

BEST: Barcode Enabled Sequencing of Tetrads

Published on: May 1, 2014

10.6K
Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella
07:11

Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella

Published on: May 13, 2019

10.2K

Area of Science:

  • Biodiversity research
  • Molecular biology
  • Conservation science

Background:

  • Accurate identification and cataloging of global biodiversity are crucial for conservation efforts.
  • Existing methods for biodiversity assessment can be costly and time-consuming.
  • The International Barcode of Life initiative has made significant progress but faces scalability challenges.

Purpose of the Study:

  • To present a novel approach, Closed-Tube Barcoding, for enhancing the speed and reducing the cost of biodiversity identification, cataloging, and monitoring.
  • To leverage existing Polymerase Chain Reaction (PCR)-based technologies for practical biodiversity assessment.
  • To support large-scale screening of numerous small or rare specimens.

Main Methods:

  • Utilizing a suite of proven PCR-based technologies.
  • Implementing a closed-tube system for streamlined specimen processing.
  • Focusing on automation and portability for widespread application.

Main Results:

  • Demonstrated a new approach for increasing the rate of biodiversity identification.
  • Showcased a method for lowering the cost of cataloging and monitoring global biodiversity.
  • Highlighted the suitability of the approach for handling large numbers of small or rare specimens.

Conclusions:

  • Closed-Tube Barcoding offers a cost-effective and efficient solution for biodiversity assessment.
  • This method enhances the capabilities of global biodiversity initiatives like the International Barcode of Life.
  • The approach is well-suited for automated, high-throughput screening and field applications.