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Updated: Mar 12, 2026

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
Published on: April 14, 2015
Closed-Tube Barcoding
Nicky M Sirianni1,1, Huijun Yuan1,1, John E Rice1,1
1Brandeis University, Department of Biology, Waltham, MA 02453-9110, USA.
Abstract:
Here, we present a new approach for increasing the rate and lowering the cost of identifying, cataloging, and monitoring global biodiversity. These advances, which we call Closed-Tube Barcoding, are one application of a suite of proven PCR-based technologies invented in our laboratory. Closed-Tube Barcoding builds on and aims to enhance the profoundly important efforts of the International Barcode of Life initiative. Closed-Tube Barcoding promises to be particularly useful when large numbers of small or rare specimens need to be screened and characterized at an affordable price. This approach is also well suited for automation and for use in portable devices.

