Related Experiment Video
Updated: Mar 12, 2026
Preparation, Characteristics, Toxicity, and Efficacy Evaluation of the Nasal Self-Assembled Nanoemulsion Tumor Vaccine In Vitro and In Vivo
Published on: September 28, 2022
Induction of Both Local Immune Response in Mice and Protection in a Rabbit Model by Intranasal Immunization with
María Paula Del Medico Zajac1,2, Flavia Adriana Zanetti1,2, María Soledad Esusy1
11 Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria (CICVyA-INTA) , Hurlingham, Argentina .
Abstract:
In this study, we evaluated the immunogenicity and efficacy of mucosal delivery of a recombinant modified vaccinia Ankara virus (MVA) expressing the secreted version of bovine herpesvirus type 1 (BoHV-1) glycoprotein D (MVA-gDs) without addition of adjuvant in two animal models. First, we demonstrated the capability of MVA-gDs of inducing both local and systemic anti-gD humoral immune response after intranasal immunization of mice. Then, we confirmed that two doses of MVA-gDs administered intranasally to rabbits induced systemic anti-gD antibodies and conferred protection against BoHV-1 challenge. Our results show the potential of using MVA as a vector for the rational design of veterinary vaccines capable of inducing specific and protective immune responses both at local and systemic level.
