Related Experiment Video
Updated: Mar 12, 2026
High-Throughput Protein Crystallization via Microdialysis
Published on: March 3, 2023
Controlled dehydration improves the diffraction quality of two RNA crystals
HaJeung Park1, Tuan Tran2, Jun Hyuck Lee3,4
1X-ray Core Facility, The Scripps Research Institute, Scripps Florida, 130 Scripps Way, Jupiter, FL, 33458, USA. hajpark@scripps.edu.
Background:
Post-crystallization dehydration methods, applying either vapor diffusion or humidity control devices, have been widely used to improve the diffraction quality of protein crystals. Despite the fact that RNA crystals tend to diffract poorly, there is a dearth of reports on the application of dehydration methods to improve the diffraction quality of RNA crystals.
Results:
We use dehydration techniques with a Free Mounting System (FMS, a humidity control device) to recover the poor diffraction quality of RNA crystals. These approaches were applied to RNA constructs that model various RNA-mediated repeat expansion disorders.
Conclusion:
The method we describe herein could serve as a general tool to improve diffraction quality of RNA crystals to facilitate structure determinations.
