Responses of earthworms and microbial communities in their guts to Triclosan

Responses of the earthworm (Eisenia fetida) and compositions of associated microbial communities were determined after exposure to various concentrations of Triclosan (TCS) for 7 d. Concentrations of TCS were greater in intestines than in epidermis of earthworms, which suggested that earthworms accumulate TCS mainly by ingestion rather than by epidermic penetration. Exposure to TCS caused a concentration-dependent increase in activities of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) and in malondialdehyde (MDA) in E. fetida. Analyses of both the bacterial and eukaryotic community by next generation sequencing (NGS), demonstrated that TCS caused a concentration-dependent decrease in sensitive genera. While relative abundances of Pseudomonas, Stenotrophomonas, and Achromobacter were increased. Nine susceptible microbial groups were more sensitive to exposure to TCS, than were activities of enzymes in earthworms. Thus, rapid genomic measures of gut flora can be used as indicators to assess adverse effects of chemicals on earthworms.