Related Experiment Video

Updated: Mar 12, 2026

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
09:25

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae

Published on: June 2, 2021

3.4K

Arf6 controls endocytosis and polarity during asexual development of Magnaporthe oryzae

Xiaohan Zhu1,2, Tengsheng Zhou3,2, Liqiong Chen1,2

  • 1Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.

FEMS Microbiology Letters
|November 5, 2016
PubMed

Insights

ADP-ribosylation factor 6 (Arf6) is crucial for asexual development in Magnaporthe oryzae. Deleting Arf6 disrupts polarized growth, leading to abnormal fungal development and impaired endocytosis.

Area of Science:

  • Mycology
  • Cell Biology
  • Molecular Genetics

Background:

  • Asexual development in phytopathogenic fungi requires precise regulation of polarized growth.
  • ADP-ribosylation factor 6 (Arf6), a small GTPase, influences cell morphology and polarity by regulating membrane trafficking and actin cytoskeleton organization.

Purpose of the Study:

  • To functionally characterize the Arf6 homolog in Magnaporthe oryzae.
  • To investigate the role of Arf6 in fungal asexual development, focusing on polarized growth and endocytosis.

Main Methods:

  • Gene deletion (Δarf6) to create mutant strains.
  • Microscopic analysis of hyphal morphology and conidia.
  • FM4-64 uptake assay to assess endocytosis.
  • Fluorescent microscopy to visualize Arf6-GFP localization and actin cytoskeleton organization.

Main Results:

  • Δarf6 mutants exhibited hyperbranching at hyphal tips and abnormal conidia due to defective polarized growth.
  • Endocytosis was significantly impaired in Δarf6 hyphae, shown by delayed FM4-64 uptake.
  • Δarf6 conidia displayed reduced length, defects in septum formation, and abnormal nuclear distribution.
  • Actin cytoskeleton was disorganized in Δarf6 conidia, with altered actin patch distribution and reduced tropomyosin accumulation.
  • Arf6-GFP localized to the septum and potentially endocytic vesicles.

Conclusions:

  • Arf6 is essential for establishing polarity during asexual development in M. oryzae.
  • Arf6 plays a critical role in endocytosis in M. oryzae.
  • Arf6 is vital for regulating actin cytoskeleton organization, hyphal tip growth, and conidiogenesis in M. oryzae.
Keywords:
Rice blastconidiogensismembrane trafficpolarization

More Related Videos

Assay for Adhesion and Agar Invasion in S. cerevisiae04:36

Assay for Adhesion and Agar Invasion in S. cerevisiae

Published on: November 8, 2006

15.5K
Microscopy of Fission Yeast Sexual Lifecycle07:47

Microscopy of Fission Yeast Sexual Lifecycle

Published on: March 9, 2016

15.3K

Related Concept Videos

Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
3.4K
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
3.9K
Yeast Signaling01:28

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
18.4K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
3.9K
Gene Regulation During Sporulation01:17

Gene Regulation During Sporulation

Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
614
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
2.6K