Related Experiment Video
Updated: Mar 12, 2026
09:52
Measurement of Strial Blood Flow in Mouse Cochlea Utilizing an Open Vessel-Window and Intravital Fluorescence Microscopy
Published on: September 21, 2021
2.9K
Differential Canalograms Detect Outflow Changes from Trabecular Micro-Bypass Stents and Ab Interno Trabeculectomy
Hardik A Parikh1,2, Ralitsa T Loewen1, Pritha Roy1
1Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, United States.
Scientific Reports
|November 5, 2016
Summary
This study introduces a novel ex vivo training system and quantitative canalography for microincisional glaucoma surgeries. The system effectively assessed outflow enhancement, with ab interno trabeculectomy showing significant improvements.
Area of Science:
- Ophthalmology
- Surgical Innovation
- Glaucoma Research
Background:
- Microincisional glaucoma surgeries (MIGS) offer a better risk profile than traditional procedures but can be unpredictable.
- Current challenges include the lack of adequate training models for angle surgeries and intraoperative success quantification.
Purpose of the Study:
- To develop an ex vivo training system for MIGS.
- To establish a quantitative canalography method for intraoperative assessment of surgical success.
Main Methods:
- Developed a porcine ex vivo eye model for surgical training.
- Utilized a differential, quantitative canalography method with slope-adjusted fluorescence intensities to measure outflow.
- Assessed outflow enhancement following trabecular micro-bypass (TMB) implantation and ab interno trabeculectomy (AIT).
Main Results:
- TMB resulted in insignificant outflow increases across quadrants (ranging from 9% ± 3% to 24% ± 9%).
- AIT demonstrated significant outflow increases, particularly in nasal quadrants (100% ± 50% and 75% ± 28%).
- The porcine model provided realistic gonioscopy and tactile feedback, mimicking human surgical experience.
Conclusions:
- The developed ex vivo system and canalography method are effective for training and assessing MIGS.
- AIT shows greater outflow enhancement potential compared to TMB in this model.
- The model highlights potential limitations and benefits of different MIGS approaches.
