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Analyzing Platelet Subpopulations by Multi-color Flow Cytometry
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Pre and post apheresis platelet CD markers evaluation using flow cytometry.

Saleh Abdullah1, Qamar Ayub2, Muhammad Saboor3

  • 1Hematology and Blood Transfusion, Faculty of Applied Medical Sciences, Jazan University, Jazan, Saudi Arabia.

JPMA. the Journal of the Pakistan Medical Association
|November 5, 2016
PubMed
Summary
This summary is machine-generated.

Apheresis procedures significantly increase platelet activation markers CD62p and CD63 on platelet surfaces. However, CD41 and CD61 expression remains unchanged, indicating specific activation pathways during apheresis.

Keywords:
Platelet, CD markers, Plateletapheresis, Flowcytometry.

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Area of Science:

  • Hematology
  • Transfusion Medicine
  • Immunology

Background:

  • Platelet activation is crucial in hemostasis and thrombosis.
  • Apheresis is a common procedure for collecting blood components, including platelets.
  • Understanding changes in platelet surface markers post-apheresis is vital for transfusion safety and efficacy.

Purpose of the Study:

  • To evaluate the impact of apheresis on the expression of platelet activation-dependent glycoproteins.
  • To assess changes in specific Cluster of Differentiation (CD) markers on platelets after apheresis.

Main Methods:

  • Collected paired venous blood and apheresis platelet samples from 100 donors.
  • Analyzed platelet CD markers (CD41, CD61, CD62p, CD63) using flow cytometry within 2 hours of collection.
  • Utilized SPSS 20 for statistical analysis of marker expression.

Main Results:

  • No significant difference in CD41 and CD61 expression between pre- and post-apheresis samples (p=0.447, p=0.712).
  • Highly significant increase in CD62p expression post-apheresis (9.57% to 55.57%, p<0.001).
  • Significant increase in CD63 expression post-apheresis (14.19% to 40.77%, p=0.04).
  • Moderate correlation observed between post-apheresis CD62p and CD63 expression (r=0.62).

Conclusions:

  • Apheresis procedure leads to increased expression of platelet activation markers CD62p and CD63.
  • CD41 and CD61 expression are not affected by the apheresis process.
  • These findings highlight specific platelet activation during apheresis, relevant for transfusion practices.