Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Formation of Species01:31

Formation of Species

46.5K
Speciation describes the formation of one or more new species from one or sometimes multiple original species. The resulting species are discrete from the parent species, and barriers to reproduction will typically exist. There are two primary mechanisms, speciation with and without geographic isolation—allopatric and sympatric speciation, respectively.
46.5K
Yeast Signaling01:28

Yeast Signaling

18.4K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
18.4K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

7.2K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
7.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evolution: Paralog interference stabilizes gene duplicates.

Current biology : CB·2026
Same author

Splicing regulation and intron evolution in the short-intron ciliate model of endosymbiosis Paramecium bursaria.

Nucleic acids research·2026
Same author

Complete end-to-end learning from protein feature representation to protein interactome inference.

GigaScience·2025
Same author

Environment-dependent mutualism-parasitism transitions in the incipient symbiosis between Tetrahymena utriculariae and Micractinium tetrahymenae.

The ISME journal·2025
Same author

Protein moonlighting by a target gene dominates phenotypic divergence of the Sef1 transcriptional regulatory network in yeasts.

Nucleic acids research·2024
Same author

Histone deacetylase Hos2 regulates protein expression noise by potentially modulating the protein translation machinery.

Nucleic acids research·2024

Related Experiment Video

Updated: Mar 12, 2026

Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae
05:39

Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae

Published on: December 2, 2022

3.3K

Experimental Evolution Reveals Interplay between Sch9 and Polyploid Stability in Yeast.

Yi-Jin Lu1,2, Krishna B S Swamy2, Jun-Yi Leu2

  • 1Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Taipei, Taiwan.

Plos Genetics
|November 5, 2016
PubMed
Summary

Robust tetraploidy in yeast is achieved by increasing the protein kinase Sch9. This key finding illuminates the early stages of polyploid genome evolution and stability.

More Related Videos

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
07:55

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

Published on: March 7, 2019

8.6K
Author Spotlight: Enhanced Isolation of Interaction-Null Mutants in Yeast
02:44

Author Spotlight: Enhanced Isolation of Interaction-Null Mutants in Yeast

Published on: December 29, 2023

1.0K

Related Experiment Videos

Last Updated: Mar 12, 2026

Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae
05:39

Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae

Published on: December 2, 2022

3.3K
A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
07:55

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

Published on: March 7, 2019

8.6K
Author Spotlight: Enhanced Isolation of Interaction-Null Mutants in Yeast
02:44

Author Spotlight: Enhanced Isolation of Interaction-Null Mutants in Yeast

Published on: December 29, 2023

1.0K

Area of Science:

  • Evolutionary biology
  • Genetics
  • Molecular biology

Background:

  • Polyploidization significantly impacts eukaryotic evolution across fungi, plants, and animals.
  • Newly formed polyploid genomes are inherently unstable, often leading to aneuploidy or diploidy.
  • The transition from unstable polyploidy to stable states remains poorly understood.

Purpose of the Study:

  • To investigate the mechanisms underlying the transition between unstable and stable polyploidy.
  • To identify key genetic factors involved in maintaining robust tetraploidy during early evolution.
  • To elucidate the role of specific protein kinases in polyploid genome stability.

Main Methods:

  • Laboratory evolution experiments were performed on yeast cells.
  • Analysis involved manipulating the abundance of the protein kinase Sch9 and TOR1.
  • Phenotypic characterization of evolved tetraploid yeast lines under stress conditions.

Main Results:

  • Increased abundance of Sch9 protein kinase promotes robust tetraploidy in evolved yeast.
  • Overexpression of SCH9, but not TOR1, confers evolved phenotypes to new tetraploids.
  • Knocking out SCH9 reduces or eliminates evolved phenotypes in tetraploid yeast.
  • Tetraploidy was maintained in evolved lines even when challenged with conditions inducing aneuploidy in ancestral cells.

Conclusions:

  • Sch9 plays a critical role in stabilizing newly formed polyploid genomes during early evolution.
  • Targeting Sch9 can facilitate the transition to robust tetraploidy, overcoming genomic instability.
  • This study provides insights into the molecular mechanisms governing polyploid genome evolution and stability.