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Related Concept Videos

The Ras Gene02:38

The Ras Gene

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The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
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The Ras Gene02:38

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Replicative Cell Senescence02:15

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Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
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Related Experiment Video

Updated: Mar 12, 2026

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence
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Senescence Phenotypes Induced by Ras in Primary Cells.

Lena Lau1, Gregory David2

  • 1Department of Biochemistry and Molecular Pharmacology, Perlmutter Cancer Institute, New York University School of Medicine, 550 First Avenue, New York, NY, 10016, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 5, 2016
PubMed
Summary

Cellular senescence is a stable cell-cycle arrest. This study details common assays for detecting oncogenic Ras-induced senescence, as a universal marker remains elusive.

Keywords:
OncogenePrimary fibroblastsRasSA-βgalSAHFSASPSenescence

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Area of Science:

  • Cellular and Molecular Biology
  • Oncology
  • Aging Research

Background:

  • Cellular senescence is a fundamental biological process characterized by stable cell-cycle arrest.
  • Various stressors, including telomere shortening and oncogene activation, can trigger senescence.
  • Senescence phenotypes are heterogeneous, making universal identification challenging.

Purpose of the Study:

  • To describe common assays for assessing oncogenic Ras-induced senescence.
  • To highlight the need for multiple markers due to the lack of a definitive senescence marker.

Main Methods:

  • Review and description of established assays for senescence detection.
  • Focus on methods applicable to oncogenic Ras-induced senescence models.

Main Results:

  • No single, universally specific marker for all senescent cells currently exists.
  • Multiple independent markers are required to reliably identify senescent cells.
  • Specific assays are effective for evaluating senescence induced by oncogenic Ras.

Conclusions:

  • Assessing cellular senescence, particularly oncogenic Ras-induced senescence, requires a multi-marker approach.
  • Further research may lead to more specific senescence detection methods.