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Related Experiment Video

Updated: Mar 12, 2026

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

Published on: July 12, 2022

Autophagy Detection During Oncogene-Induced Senescence Using Fluorescence Microscopy.

Masako Narita¹, Masashi Narita²

¹Cancer Research UK Cambridge Institute, University of Cambridge, Robinson Way, Cambridge, CB2 0RE, UK.

Methods in Molecular Biology (Clifton, N.J.)

|November 5, 2016

Summary

Oncogene-induced senescence (OIS) involves multiple effectors, including autophagy. This study details generating OIS in human diploid fibroblasts and detecting autophagy using immunofluorescence, aiding senescence research.

Keywords:

Autophagy Immunofluorescence Lysosome Senescence mTOR

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Area of Science:

Cell Biology
Cancer Research
Molecular Biology

Background:

Oncogene-induced senescence (OIS) is a dynamic process with various effector mechanisms influencing its phenotype.
Autophagy, a cellular degradation pathway, is implicated as a senescence effector, but its role is context-dependent.

Purpose of the Study:

To describe the generation of OIS in human diploid fibroblasts (HDFs).
To detail methods for detecting autophagy in OIS models, focusing on immunofluorescence techniques.

Main Methods:

Generation of OIS using human diploid fibroblasts (HDFs).
Detection of autophagy via immunofluorescence microscopy.
Utilizing established protocols for monitoring cellular processes.

Main Results:

Successful generation of OIS in HDFs.
Demonstration of immunofluorescence-based autophagy detection in OIS models.
Provides a reproducible methodology for studying OIS and autophagy.

Conclusions:

Human diploid fibroblasts serve as a robust model for studying OIS.
Immunofluorescence is a key technique for visualizing and quantifying autophagy in OIS.
This chapter offers practical guidance for researchers investigating the interplay between OIS and autophagy.