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Oligopeptide Competition Assay for Phosphorylation Site Determination
Published on: May 18, 2017
AMPK in Yeast: The SNF1 (Sucrose Non-fermenting 1) Protein Kinase Complex
Pascual Sanz1, Rosa Viana2, Maria Adelaida Garcia-Gimeno3
1Instituto de Biomedicina de Valencia, CSIC and Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER-ISCiii), Jaime Roig 11, 46010, Valencia, Spain. sanz@ibv.csic.es.
Abstract:
In yeast, SNF1 protein kinase is the orthologue of mammalian AMPK complex. It is a trimeric complex composed of Snf1 protein kinase (orthologue of AMPKα catalytic subunit), Snf4 (orthologue of AMPKγ regulatory subunit), and a member of the Gal83/Sip1/Sip2 family of proteins (orthologues of AMPKβ subunit) that act as scaffolds and also regulate the subcellular localization of the complex. In this chapter, we review the recent literature on the characteristics of SNF1 complex subunits, the structure and regulation of the activity of the SNF1 complex, its role at the level of transcriptional regulation of relevant target genes and also at the level of posttranslational modification of targeted substrates. We also review the crosstalk of SNF1 complex activity with other key protein kinase pathways such as cAMP-PKA, TORC1, and PAS kinase.
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