Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microorganisms in Medicine and Therapeutics

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.

Gene Therapy

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...

Bacterial Transformation

Bacterial Transformation

Bacterial Transformation

Bacterial Transformation

In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...

Transduction

Transduction

Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome...

Recombinant DNA

Recombinant DNA

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Formyl peptide receptor 1 agonism selectively suppresses mitochondria-driven neutrophil extracellular trap formation while preserving antibacterial immunity.

Molecular medicine (Cambridge, Mass.)·2026

Same author

Neutrophil extracellular traps in the animal model of adenine-induced chronic kidney disease.

PloS one·2026

Same author

Selective mitochondrial dysfunction and redox collapse induced by an indole chalcone in breast cancer cells.

Chemico-biological interactions·2026

Same author

Cisplatin - induced peripheral neuropathy and biomarkers of gut microbial translocation in testicular germ cell tumor survivors.

Frontiers in immunology·2026

Same author

Neonatal testosterone exposure modulates exploration and object avoidance without exacerbating autism-like behavior in Shank3b-deficient mice.

Hormones and behavior·2026

Same author

Extracellular heme:DNA complexes promote oxidative stress and inflammation during lupus-associated hemolysis.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

AKT-mTOR/P53 PathwayDriven RapamycinAlpelisib Efficacy in Animal Models of TIE2Mutant Venous Malformations.

Frontiers in bioscience (Landmark edition)·2026

Same journal

Dual Role of Nitric Oxide in Tumor Immunity and Its Therapeutic Application.

Frontiers in bioscience (Landmark edition)·2026

Same journal

The Metabolic States of Cancer-Associated Fibroblasts: Targeting Stromal Reprogramming to Impede Tumor Progression and Immune Evasion.

Frontiers in bioscience (Landmark edition)·2026

Same journal

Functional Changes in Mitochondrial Subpopulations of Left Ventricular Cardiomyocytes in Post-Infarction Rats During the Subacute Stage of Remodeling.

Frontiers in bioscience (Landmark edition)·2026

Same journal

TRPM7 Channel-Mediated Mitochondrial Oxidative Stress Induces Dysfunction of Müller Cells Under High Glucose and Low Mg<sup>2+</sup> Stress.

Frontiers in bioscience (Landmark edition)·2026

Same journal

Navigating the Redox Precipice: Metabolic Gatekeeping as a Therapeutic Window in Pancreatic Precancer.

Frontiers in bioscience (Landmark edition)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 12, 2026

Bioluminescent Bacterial Imaging In Vivo

Bioluminescent Bacterial Imaging In Vivo

Published on: November 4, 2012

Gene therapy using bacterial vectors.

Peter Celec¹, Roman Gardlik²

¹Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Sasinkova 4, 811 08 Bratislava, Slovakia, petercelec@gmail.com.

Frontiers in Bioscience (Landmark Edition)

|November 5, 2016

Summary

This summary is machine-generated.

Bacteria offer two gene therapy strategies: bactofection for gene transfer and alternative methods for controlled protein delivery. Both show promise but require improved safety and efficiency for clinical use.

More Related Videos

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Published on: January 7, 2019

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

Related Experiment Videos

Last Updated: Mar 12, 2026

Bioluminescent Bacterial Imaging In Vivo

Bioluminescent Bacterial Imaging In Vivo

Published on: November 4, 2012

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Published on: January 7, 2019

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

Area of Science:

Biotechnology
Microbiology
Gene Therapy

Background:

Bacteria present novel strategies for gene therapy, including bactofection and alternative prokaryotic expression systems.
These methods offer unique advantages for applications like gene substitution, DNA vaccination, and targeted protein delivery.

Purpose of the Study:

To review the advantages and challenges of using bacterial vectors in gene therapy.
To summarize current applications of bacteria-mediated gene therapy in biomedical research.

Main Methods:

Review of existing literature on bacterial gene therapy strategies.
Analysis of bactofection and alternative gene therapy approaches.
Examination of transkingdom RNA interference as a specific application.

Main Results:

Bactofection is suitable for gene substitution and DNA vaccination.
Alternative gene therapy excels at in situ protein delivery and intracellular treatments.
Bacterial vectors have shown potential in treating solid tumors and gut infections.

Conclusions:

Bacterial gene therapy offers diverse applications but faces challenges in clinical translation.
Significant improvements in safety and efficiency are necessary for widespread clinical adoption.