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Related Concept Videos

Mitosis and Cytokinesis02:03

Mitosis and Cytokinesis

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In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome duplication, chromosome distribution to daughter cells, and finally, cell division. The cell cycle is tightly regulated by its regulatory systems as well as extracellular signals that affect cell proliferation.
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...
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In eukaryotes, the cell division cycle is divided into distinct, coordinated cellular processes that include cell growth, DNA replication/chromosome duplication, chromosome distribution to daughter cells, and finally, cell division. The cell cycle is tightly regulated by its regulatory systems as well as extracellular signals that affect cell proliferation.
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The Mitotic Spindle02:27

The Mitotic Spindle

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The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
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Meiosis vs. Mitosis02:57

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Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
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Meiosis II02:02

Meiosis II

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Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
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Related Experiment Video

Updated: Mar 12, 2026

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

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Large-Scale Mitotic Cell Synchronization.

Kalyan Dulla1,2, Anna Santamaria Margalef3

  • 1Department of Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|November 6, 2016
PubMed
Summary

Synchronizing cell populations is crucial for studying cell cycle regulation. This chapter details methods for large-scale isolation of mitotic mammalian cells, optimizing enrichment at specific cell cycle stages.

Keywords:
Anaphase/telophaseHeLa SHeLa S3Large-scale synchronizationMetaphaseMitosisPrometaphaseSpinner culturesTriple flasks

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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
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Area of Science:

  • Cell Biology
  • Molecular Biology

Background:

  • Cell cycle regulation governs cell growth and division.
  • Accurate study necessitates synchronized cell populations at specific phases.
  • Existing synchronization methods include serum deprivation, contact inhibition, centrifugal elutriation, and drug-dependent methods.

Purpose of the Study:

  • To describe protocols for large-scale isolation of mitotic mammalian cells.
  • To detail technical aspects of adherent and suspension cell isolation.
  • To present methods for enriching cells at distinct mitotic stages with optimized culture conditions.

Main Methods:

  • Drug-dependent synchronization for cell cycle enrichment.
  • Large-scale isolation techniques for mitotic mammalian cells.
  • Optimization of culture conditions for specific mitotic stages.

Main Results:

  • Established protocols for isolating mitotic mammalian cells in large quantities.
  • Demonstrated methods for enriching cells at various mitotic stages.
  • Optimized culture conditions to support cell cycle progression studies.

Conclusions:

  • Effective synchronization protocols are vital for cell cycle research.
  • The described methods facilitate large-scale isolation and enrichment of mitotic cells.
  • Optimized conditions enhance the study of cell cycle regulatory events.