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Updated: Mar 12, 2026
Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
Published on: July 22, 2012
Molecular detection of human parechovirus in under-Five-Year-Old Children with gastroenteritis
Massimiliano Bergallo1, Ilaria Galliano1, Paola Montanari1
1Department of Public Health and Pediatric Sciences, Citoimmunodiagnostics Laboratory, University of Turin, Medical School, 10136 Turin, Italy; SC. Pediatric U Regina Margherita Hospital, Piazza Polonia 94, 10126 Turin, Italy.
Background:
Currently, RT-PCR is used widely and considered to be a convenient, useful, and powerful method for molecular diagnosis, to detect pathogens from clinical specimens.
Objectives:
In this work we describe the development of an in-house Real-time Taqman PCR assay for quantification of HPeV in stool specimens.
Study Designs:
A total of 137 fecal specimens previously screened for rotavirus and adenovirus were tested for HPeV virus.
Results:
A total of 11 out of 137 (8%) episodes of acute gastroenteritis were associated with HPeV genomic detection with median viral load 14678±28927 genomes/mg fecal specimens. There was no significant difference in the detection rate between male and female (54.5% (6/11) vs. 45.5% (5/11). Among the 11 HPeV-positive cases, 2 were also positive for other viral pathogens, including rotavirus (n=2).
Conclusion:
In conclusion, the development of a laboratory designed Real Time PCR TaqMan assay for quantitative detection of HPeV and the optimization and standardization of this assay using stool of children with acute gastroenteritis are described.
