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Enzyme-linked, aptamer-based, competitive biolayer interferometry biosensor for palytoxin.

Shunxiang Gao1, Xin Zheng2, Bo Hu3

  • 1Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China; Research Center, Eye & ENT Hospital, Fudan University, Shanghai, 200032 China; Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai 200032, China; Key Laboratory of Myopia, Ministry of Health, Fudan University, Shanghai 200032, China.

Biosensors & Bioelectronics
|November 7, 2016
PubMed
Summary

A new biosensor uses enzyme-linked aptamers and biolayer interferometry for rapid, sensitive detection of marine biotoxin palytoxin. This method offers a promising tool for real-time toxin analysis in various samples.

Keywords:
AptamerBiolayer interferometryBiosensorPalytoxinSELEX

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Environmental Science

Background:

  • Marine biotoxins, such as palytoxin, pose significant risks to human health and marine ecosystems.
  • Accurate and rapid detection methods are crucial for monitoring and managing seafood safety.

Purpose of the Study:

  • To develop a real-time, ultra-sensitive, and rapid quantitative method for palytoxin detection.
  • To create an enzyme-linked aptamer-based biosensor utilizing biolayer interferometry (BLI).

Main Methods:

  • Coupling biolayer interferometry (BLI) with a competitive binding assay.
  • Utilizing horseradish peroxidase-labeled aptamers as biorecognition receptors.
  • Immobilizing palytoxin on a biosensor surface and detecting binding events via optical thickness changes.

Main Results:

  • Achieved a broad linear range for palytoxin detection (200-700 pg/mL).
  • Demonstrated a low detection limit of 0.04 pg/mL.
  • Showcased high selectivity, reproducibility, and stability in spiked sample analysis.

Conclusions:

  • The developed enzyme-linked, aptamer-based, competitive BLI biosensor provides a sensitive and rapid method for palytoxin detection.
  • This approach holds potential for the detection of other analytes.
  • Offers a promising tool for marine biotoxin monitoring and food safety applications.