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A pilot study on interaction between donkey tetherin and EIAV stains with different virulent and replication
Qiucheng Yao1, Jian Ma2, Xuefeng Wang2
1College of Veterinary Medicine, Northeast Agricultural University, Harbin, China; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin, China.
Microbial Pathogenesis
|November 7, 2016
Summary
Donkey tetherin (do-tetherin) restricts equine infectious anemia virus (EIAV) release, but EIAV strains evolve to counteract this host restriction differently, influencing viral evolution.
Area of Science:
- Virology
- Immunology
- Host-Pathogen Interactions
Background:
- Tetherin (BST-2) is a host restriction factor inhibiting enveloped virus release.
- Equine infectious anemia virus (EIAV) has evolved countermeasures to host restriction factors.
- The role of host restriction factors in EIAV evolution during serial passage is unclear.
Purpose of the Study:
- To investigate the differential adaptation of EIAV strains to donkey tetherin (do-tetherin).
- To determine if EIAV virulence correlates with its ability to overcome do-tetherin restriction.
Main Methods:
- Generation of distinct EIAV strains (EIAVDV, EIAVDLV, EIAVFDDV) through serial passage.
- Assessment of do-tetherin's inhibitory effect on viral particle release for each strain.
- Evaluation of EIAV envelope protein (Env) mediated counteraction of do-tetherin restriction.
Main Results:
- Donkey tetherin inhibited the release of all three EIAV strains, with varying intensity (EIAVDV < EIAVDLV < EIAVFDDV).
- All EIAV strains counteracted do-tetherin restriction via their Env proteins, with differing efficiencies (EIAVDV > EIAVDLV > EIAVFDDV).
Conclusions:
- Donkey tetherin plays a significant role in shaping EIAV evolution during serial passage.
- Differential adaptation to host restriction factors contributes to the distinct characteristics of EIAV strains.
