Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

9.9K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
9.9K
Export of Mitochondrial and Chloroplast Genes02:19

Export of Mitochondrial and Chloroplast Genes

4.4K
A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred...
4.4K
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

812
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
812

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Internal validation of the fully continuous model in EuroForMix for its implementation in routine forensic DNA profiling.

Forensic science international. Genetics·2026
Same author

PACIC: patient activation and problem solving not related to age in patients with depressive symptoms.

European archives of psychiatry and clinical neuroscience·2025
Same author

Evaluating the effect of marker panel sizes on estimation of bio-geographical co-ancestry proportions.

Forensic science international. Genetics·2025
Same author

Suboptimal antidepressant use among inpatients and outpatients with symptoms of depression: a cross-sectional analysis of the POKAL core data set.

European archives of psychiatry and clinical neuroscience·2025
Same author

Inter-platform evaluation of the MPSplex large-scale tri-allelic SNP panel for forensic identification.

Forensic science international. Genetics·2025
Same author

A CE-based mRNA profiling method including six targets to estimate the time since deposition of blood stains.

Forensic science international. Genetics·2025

Related Experiment Video

Updated: Mar 12, 2026

Author Spotlight: High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution
10:47

Author Spotlight: High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

Published on: May 5, 2023

4.7K

A collaborative EDNAP exercise on SNaPshot™-based mtDNA control region typing.

N E C Weiler1, K Baca2, D Ballard3

  • 1Netherlands Forensic Institute, The Hague, The Netherlands.

Forensic Science International. Genetics
|November 7, 2016
PubMed
Summary
This summary is machine-generated.

The mini-mtSNaPshot assay effectively profiles mitochondrial DNA (mtDNA) SNPs for haplogroup analysis, achieving a 97.6% success rate across 15 European labs. Minor interpretation errors highlight the need for targeted training and guidelines.

Keywords:
Forensic scienceHaplogroupMassively parallel sequencingSNaPshotmtDNA

More Related Videos

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
07:24

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

2.0K
Methodology for Accurate Detection of Mitochondrial DNA Methylation
12:11

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Published on: May 20, 2018

14.0K

Related Experiment Videos

Last Updated: Mar 12, 2026

Author Spotlight: High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution
10:47

Author Spotlight: High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

Published on: May 5, 2023

4.7K
Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
07:24

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

2.0K
Methodology for Accurate Detection of Mitochondrial DNA Methylation
12:11

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Published on: May 20, 2018

14.0K

Area of Science:

  • Forensic Genetics
  • Mitochondrial DNA Analysis
  • Population Genetics

Background:

  • The European DNA Profiling (EDNAP) Group evaluated the mini-mtSNaPshot assay.
  • This assay targets 18 single nucleotide polymorphism (SNP) positions in the mitochondrial DNA (mtDNA) control region.
  • The assay is designed to discriminate major European mtDNA haplogroups.

Purpose of the Study:

  • To assess the performance and reliability of the mini-mtSNaPshot screening assay.
  • To evaluate the feasibility of haplogroup assignment and phylogenetic consistency using 18 mtDNA SNPs.
  • To identify potential challenges and areas for improvement in assay application within forensic laboratories.

Main Methods:

  • A collaborative exercise involving 15 European forensic genetics laboratories.
  • Analysis of 13 diverse samples, including single-source, mixed, and degraded samples.
  • Comparison of SNP typing data against standard mtDNA reference profiles.

Main Results:

  • An overall success rate of 97.6% in obtaining useful results was achieved.
  • Most participants could infer haplotypes and assess phylogenetic consistency.
  • A small percentage (2%) of incorrect comparisons indicated a need for improved data interpretation guidelines.

Conclusions:

  • The mini-mtSNaPshot assay demonstrates high performance for mtDNA haplogroup analysis in a collaborative forensic setting.
  • While generally reliable, specific training and clear guidelines are necessary for accurate interpretation of mini-mtSNaPshot data.
  • The assay shows promise for routine forensic casework involving mtDNA analysis.