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Absorption of Nasal and Bronchial Fluids: Precision Sampling of the Human Respiratory Mucosa and Laboratory Processing of Samples
Published on: January 21, 2018
[An epidemiological study on human rhinovirus C in hospitalized children with respiratory tract infections]
1Department of Respiratory Medicine, Children's Hospital Affiliated to Soochow University, Suzhou, Jiangsu 215003, China. yyd3060@126.com.
Insights
Human rhinovirus C (HRV-C) causes nearly one-third of respiratory tract infections in hospitalized children in Suzhou, China. This common viral infection peaks in autumn, particularly affecting children aged 2-5 years.
Area of Science:
- Virology
- Pediatric Infectious Diseases
- Respiratory Medicine
Background:
- Human Rhinovirus (HRV) is a common cause of respiratory tract infections (RTIs).
- Human Rhinovirus C (HRV-C) is a distinct species known to cause significant respiratory illness.
- Understanding HRV-C epidemiology and clinical impact in pediatric populations is crucial for public health.
Purpose of the Study:
- To determine the detection rates of HRV-C in hospitalized children with RTIs in Suzhou, China.
- To analyze the epidemiological characteristics, including seasonality and age distribution, of HRV-C infections.
- To investigate the clinical features and co-infection patterns associated with HRV-C.
Main Methods:
- A cohort of 1,702 hospitalized children with RTIs was studied from January to December 2014.
- Nasopharyngeal aspirate samples were collected for viral detection.
- Reverse transcription-polymerase chain reaction (RT-PCR) and quantitative real-time PCR with high-resolution melting were used to identify HRV and HRV-C.
Main Results:
- HRV was detected in 14.34% of children, with HRV-C accounting for 28.3% of these HRV infections.
- HRV-C exhibited year-round circulation, with a significantly higher detection rate in autumn.
- Children aged 2-5 years showed a higher HRV-C detection rate; HRV-C infections were associated with lobar pneumonia, asthma exacerbation, and elevated neutrophils/CRP compared to HRV-A/B.
Conclusions:
- HRV-C is a significant contributor to pediatric RTIs, representing approximately one-third of all HRV infections.
- High rates of co-infection with other viruses and bacteria were observed in HRV-C cases.
- HRV-C infections present with distinct clinical outcomes, including increased severity markers and specific respiratory conditions.
Objective:
To investigate the detection rates, epidemical characteristics, and clinical features of human rhinovirus C (HRV-C) in hospitalized children with respiratory tract infections (RTIs) in Suzhou, China.
Methods:
A total of 1 702 hospitalized children with RTIs from January to December, 2014 were enrolled, and 1 702 nasopharyngeal aspirate samples were collected from all children. RT-PCR was used to measure HRV mRNA, and quantitative real-time PCR combined with high-resolution melting curve was used to measure HRV-C.
Results:
Of all children, 244 (14.34%) were detected to have HRV infection, among whom 69 (69/244, 28.3%) had HRV-C infection. The rate of mixed infection of HRV-C with other viruses and bacteria was 61% (42/69). HRV-C was detected in each month of the year, and the detection rate of HRV-C in autumn was significantly higher than that in spring, summer, and winter (P<0.05). The children aged 2-5 years had a significantly higher detection rate of HRV-C than those in the other age groups (P<0.05). Compared with HRV-A/B infection, HRV-C infection led to significantly higher proportions of patients with lobar pneumonia and acute exacerbation of asthma (P<0.05), as well as patients with increased neutrophil count and CRP level (P<0.05). There were no significant differences in sex distribution or other clinical manifestations (P>0.05).
Conclusions:
HRV-C infection accounts for about 1/3 of HRV infection, with a high incidence rate in autumn. The rate of mixed infection of HRV-C with other viruses and bacteria is high, and children aged 2-5 years have the highest detection rate of HRV-C. Children with HRV-C infection have similar clinical manifestations as those with HRV-A/B infection.
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