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Related Experiment Video

Updated: Mar 12, 2026

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
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Mitochondrial Isolation and Purification from Mouse Spinal Cord.

Philippe A Parone1, Sandrine Da Cruz1, Don W Cleveland1

  • 1Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California at San Diego (UCSD), La Jolla, CA, USA.

Bio-Protocol
|November 8, 2016
PubMed
Summary

This study details a new protocol for isolating mitochondria from mouse spinal cord tissue. This method is crucial for studying mitochondrial dysfunction in neurological diseases like amyotrophic lateral sclerosis.

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Area of Science:

  • Cell Biology
  • Neuroscience
  • Mitochondrial Biology

Background:

  • Mitochondria are vital organelles involved in cellular energy production and calcium homeostasis.
  • Mitochondrial dysfunction is linked to neurodegenerative diseases, including Parkinson's, Alzheimer's, and ALS.
  • Studying specific brain regions affected by disease requires specialized isolation techniques.

Purpose of the Study:

  • To present a reliable protocol for isolating mitochondria from mouse spinal cord.
  • To facilitate research into mitochondrial function in the context of neuromuscular diseases.

Main Methods:

  • Isolation of mitochondria from mouse spinal cord.
  • Utilizing differential centrifugation for separating mitochondria from other cellular components.

Main Results:

  • Successfully isolated intact mitochondria from mouse spinal cord tissue.
  • The protocol provides a method for obtaining mitochondria for further functional analysis.

Conclusions:

  • The described protocol enables the study of mitochondrial function in the spinal cord.
  • This method supports research into the role of mitochondria in diseases like ALS.