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Multiplex Flow Assays.
Kristofer J Haushalter1, Srinivas Vetcha1, Robert C Haushalter1
1Parallel Synthesis Technologies, Inc. , 3054 Lawrence Expwy., Santa Clara, California 95051, United States.
ACS Omega
|November 8, 2016
Summary
Researchers developed a novel multiplex assay to simultaneously detect numerous analytes, overcoming limitations of traditional lateral flow assays. This method uses optically encoded beads for versatile, high-throughput diagnostic testing.
Area of Science:
- Biotechnology
- Analytical Chemistry
- Diagnostics
Background:
- Lateral flow assays are widely used but limited to detecting one or few analytes.
- Current diagnostic methods struggle with simultaneous detection of multiple targets.
Purpose of the Study:
- To develop a general method for transforming single-analyte lateral flow assays into multiplex assays.
- To enable simultaneous detection of an arbitrarily large number of analytes.
Main Methods:
- Developed optically encoded agarose beads with unique lanthanide emitter signatures for capture probes.
- Integrated encoded beads into lateral flow assays, capturing analytes via specific probes.
- Detected captured analytes using fluorescent reporters for multiplex analysis.
Main Results:
- Successfully demonstrated simultaneous detection of DNA, antigen-antibody pairs, and multiple nucleic acids/antibodies.
- Transformed single-plex lateral flow assays into versatile multiplex diagnostic platforms.
- Achieved high-throughput analysis by identifying analytes via optical codes on beads.
Conclusions:
- The novel method offers a generalizable approach to multiplexing lateral flow assays.
- This technique significantly enhances diagnostic capabilities for various applications.
- Optically encoded beads provide a robust platform for simultaneous analyte detection.
