Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Biological Methods for Microbial Control01:28

Biological Methods for Microbial Control

1.2K
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
1.2K
Antimicrobial Proteins01:23

Antimicrobial Proteins

15.2K
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
15.2K
Chemical Agents for Microbial Control01:27

Chemical Agents for Microbial Control

1.4K
Chemicals play important roles in controlling microbial growth by targeting microbial structures and functions as sanitizers, antiseptics, disinfectants, and sterilants.Alcohols are commonly used sanitizers, effectively disrupting lipid membranes, which compromises cell integrity. They are also used as antiseptics and disinfectants due to their rapid action and versatility.Phenols and their derivatives phenolics , known for denaturing proteins and disrupting cell membranes, are particularly...
1.4K
Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

3.3K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
3.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nanobody-mediated control of long RSH Rel and RelA catalysis by restriction of their conformational landscape.

Nature communications·2026
Same author

Nuclease-NTPase antiphage defence systems use conserved molecular features to control bacterial immunity.

Nature microbiology·2026
Same author

Phage homing endonuclease amplifies anti-defense genes to evade bacterial immunity.

Nature communications·2026
Same author

Nanomotion-enabled ultra-rapid antibiotic susceptibility testing with magnetic bead-based pathogen enrichment for accelerated sepsis diagnostics.

Scientific reports·2026
Same author

Structural and functional diversity of toxin-antitoxin-chaperone systems.

Cell reports·2026
Same author

Characterization of five environmental phages infecting <i>Escherichia coli</i> K-12 isolated during a phage biology training course.

Microbiology spectrum·2025

Related Experiment Video

Updated: Mar 12, 2026

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
11:56

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Published on: May 4, 2018

13.1K

Cationic bactericidal peptide 1018 does not specifically target the stringent response alarmone (p)ppGpp.

Liis Andresen1,2, Tanel Tenson3, Vasili Hauryliuk1,2,3

  • 1Department of Molecular Biology, Umeå University, Building 6K, 6L University Hospital Area, SE-901 87 Umeå, Sweden.

Scientific Reports
|November 8, 2016
PubMed
Summary

Synthetic peptide 1018 does not specifically target the stringent response or biofilms. Its antimicrobial activity is independent of the (p)ppGpp messenger, challenging previous findings.

More Related Videos

Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization
10:13

Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization

Published on: August 11, 2018

12.9K
Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis
09:26

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis

Published on: May 23, 2021

3.7K

Related Experiment Videos

Last Updated: Mar 12, 2026

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
11:56

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Published on: May 4, 2018

13.1K
Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization
10:13

Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization

Published on: August 11, 2018

12.9K
Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis
09:26

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis

Published on: May 23, 2021

3.7K

Area of Science:

  • Microbiology
  • Bacterial Physiology
  • Antimicrobial Research

Background:

  • The bacterial stringent response, regulated by (p)ppGpp, controls virulence, biofilm formation, and antibiotic tolerance.
  • This response is a potential target for novel antibacterial agents.
  • Synthetic peptide 1018 was proposed to inhibit the stringent response and disrupt biofilms by interacting with (p)ppGpp.

Purpose of the Study:

  • To investigate the specificity of peptide 1018's proposed mechanism of action.
  • To determine if peptide 1018 specifically targets the stringent response and biofilm formation.

Main Methods:

  • Comparative analysis of peptide 1018 and control peptide 8101 on Pseudomonas aeruginosa planktonic and biofilm growth.
  • Testing peptide 1018 efficacy on Escherichia coli planktonic growth under conditions where (p)ppGpp is essential or dispensable.
  • Assessing the effect of genetic disruption of (p)ppGpp synthesis genes (relA and spoT) on E. coli sensitivity to peptide 1018.

Main Results:

  • Peptides 1018 and 8101 showed equal potency against P. aeruginosa planktonic and biofilm growth, with no preferential anti-biofilm activity.
  • Peptide 1018 inhibited E. coli planktonic growth similarly, regardless of (p)ppGpp's essentiality.
  • Disruption of (p)ppGpp synthesis genes moderately sensitized E. coli to peptide 1018, rather than protecting it.

Conclusions:

  • The antimicrobial activity of peptide 1018 is not dependent on specific recognition of the stringent response messenger (p)ppGpp.
  • The proposed mechanism of peptide 1018 specifically disrupting biofilms by inhibiting the stringent response is not supported by these findings.
  • Further research is needed to elucidate the true mechanism of action for peptide 1018 and similar compounds.