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Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications

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Published on: November 10, 2017

High efficiency upconversion nanophosphors for high-contrast bioimaging.

Masfer H Alkahtani¹, Fahad S Alghannam, Carlos Sanchez

¹The National Center for Applied Physics, KACST, PO Box 6086, Riyadh 11442, Saudi Arabia. Institute for Quantum Science and Engineering (IQSE) and Department of Physics and Astronomy, Texas A&M University, College Station, TX 77843-4242, USA.

|November 8, 2016

Summary

Upconversion nanoparticles (UCNPs) enable deep tissue bio-imaging by suppressing autofluorescence. Yttrium vanadate (YVO4) UCNPs injected into fire ants produced high-contrast 2D optical images.

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Area of Science:

Materials Science
Biotechnology
Optical Imaging

Background:

Upconversion nanoparticles (UCNPs) offer advantages over dyes for bio-imaging, including reduced autofluorescence, lower excitation thresholds, and improved photostability.
Yttrium vanadate (YVO4) doped with erbium (Er+3) and ytterbium (Yb+3) exhibits strong upconversion luminescence at low excitation intensities, making it suitable for biological applications.

Purpose of the Study:

To evaluate YVO4:Er+3, Yb+3 nanoparticles as internal probes for bio-imaging within insects.
To demonstrate the feasibility of obtaining high-contrast 2D optical images of internal biological structures.

Main Methods:

Injection of YVO4:Er+3, Yb+3 nanoparticles into fire ants (biological model).
2D optical imaging using 980 nm illumination.
Detection of upconversion fluorescence during scanned laser excitation.

Main Results:

Successful visualization of internal structures within fire ants using UCNPs.
High-contrast images with a high signal-to-noise ratio were obtained.
Demonstrated the effectiveness of UCNPs for in vivo bio-imaging in insects.

Conclusions:

YVO4:Er+3, Yb+3 nanoparticles are effective internal probes for deep tissue bio-imaging in insects.
The low excitation intensity requirement and high signal quality make these UCNPs promising for various bio-imaging applications.