Identification of degradation routes of metamitron in soil microcosms using ¹³C-isotope labeling

Metamitron is one of the most commonly used herbicide in sugar beet and flower bulb cultures. Numerous laboratory and field studies on sorption and degradation of metamitron were performed. Detailed biodegradation studies in soil using ¹³C-isotope labeling are still missing. Therefore, we aimed at providing a detailed turnover mass balance of ¹³C₆-metamitron in soil microcosms over 80 days. In the biotic system, metamitron mineralized rapidly, and ¹³CO₂ finally constituted 60% of the initial ¹³C₆-metamitron equivalents. In abiotic control experiments CO₂ rose to only 7.4% of the initial ¹³C₆-metamitron equivalents. The ¹³C label from ¹³C₆-metamitron was incorporated into microbial amino acids that were ultimately stabilized in the soil organic matter forming presumably harmless biogenic residues. Finally, ¹³C label from ¹³C₆-metamitron was distributed between the ¹³CO₂ and the ¹³C-biogenic residues indicating nearly complete biodegradation. The parallel increase of ¹³C-alanine, ¹³C-glutamate and ¹³CO₂ indicates that metamitron was initially biodegraded via the desamino-metamitron route suggesting its relevance in the growth metabolism. In later phases of biodegradation, the "Rhodococcus route" was indicated by the low ¹³CO₂ evolution and the high relevance of the pyruvate pathway, which aims at biomolecule synthesis and seems to be related to starvation. This is a first report on the detailed degradation route of metamitron in soil.