Related Experiment Video
Updated: Mar 12, 2026
09:38
Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry
Published on: June 26, 2019
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The nucleolar protein GLTSCR2 is required for efficient viral replication
Peng Wang1, Wen Meng1, Shi-Chong Han1
1Key Laboratory of Zoonosis of Ministry of Agriculture, China Agricultural University, Beijing, China.
Scientific Reports
|November 9, 2016
Summary
Glioma tumor suppressor candidate region gene 2 protein (GLTSCR2) aids viral replication by moving to the cytoplasm and weakening the innate immune response. Targeting GLTSCR2 may offer a new strategy for antiviral therapies.
Area of Science:
- Virology
- Immunology
- Molecular Biology
Background:
- The nucleolar protein Glioma tumor suppressor candidate region gene 2 protein (GLTSCR2) role in cellular innate immunity was investigated.
- Understanding GLTSCR2's function is crucial for deciphering host-pathogen interactions during viral infections.
Purpose of the Study:
- To elucidate the role of GLTSCR2 in the cellular innate immune response to viral infections.
- To determine how GLTSCR2 influences viral replication and host defense mechanisms.
Main Methods:
- Investigated GLTSCR2's cellular localization changes upon viral infection.
- Examined the interaction of cytoplasmic GLTSCR2 with RIG-I and USP15.
- Assessed the impact of GLTSCR2 translocation inhibition on viral replication and IFN-β production.
Main Results:
- GLTSCR2 supports the replication of rhabdovirus, paramyxovirus, and coronavirus.
- Viral infection induces GLTSCR2 nuclear-to-cytoplasmic translocation, where it interacts with RIG-I and USP15.
- This interaction enhances USP15 activity, leading to RIG-I deubiquitination, reduced type I interferon (IFN-β) production, and consequently, enhanced viral replication.
Conclusions:
- GLTSCR2 attenuates the host's innate immune response by interfering with the RIG-I signaling pathway.
- GLTSCR2 facilitates efficient viral replication, suggesting it as a potential therapeutic target for controlling viral infections.
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