Related Experiment Video

Updated: Mar 12, 2026

Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel
06:44

Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel

Published on: September 8, 2017

14.1K

Tristetraprolin disables prostate cancer maintenance by impairing proliferation and metabolic function

Anders E Berglund1, Kristen E N Scott2, Weimin Li2

  • 1Department of Biostatistics and Bioinformatics, H. Lee Moffitt Cancer Center & Research Institute, Tampa, Florida, USA.

Oncotarget
|November 9, 2016
PubMed

Insights

Tristetraprolin (TTP) is a tumor suppressor that indicates poor prognosis in prostate cancer. Restoring TTP function inhibits aggressive tumor cell metabolism and proliferation.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Tristetraprolin (TTP) is an RNA-binding protein that post-transcriptionally regulates gene expression.
  • TTP acts as a tumor suppressor in certain cancers, with low expression linked to reduced survival in malignancies.
  • The role of TTP in prostate cancer progression and its functional impact remain largely unexplored.

Purpose of the Study:

  • To investigate the prognostic and functional significance of Tristetraprolin (TTP) in human prostate cancer.
  • To determine the impact of TTP expression on prostate cancer cell growth, proliferation, and tumorigenic potential.
  • To elucidate the metabolic alterations associated with TTP expression in prostate tumors.

Main Methods:

  • Gene expression analysis of human prostate tumors to correlate TTP levels with clinical outcomes (Gleason score, recurrence).
  • Experimental manipulation of TTP levels in prostate cancer cell lines to assess effects on growth, proliferation, and clonogenicity.
  • In vivo studies using a mouse xenograft model to evaluate the impact of forced TTP expression on tumor formation.
  • Pathway and metabolic analyses to identify cellular processes affected by TTP.

Main Results:

  • Low TTP expression in prostate tumors correlates with high-risk Gleason scores and increased biochemical recurrence.
  • Inducible TTP expression significantly inhibits the growth, proliferation, and clonogenic potential of prostate cancer cells.
  • Forced TTP expression markedly impairs the tumorigenic capacity of prostate cancer cells in vivo.
  • TTP expression alters cellular metabolism in prostate tumor cells, impairing key processes like mitochondrial respiration.

Conclusions:

  • Tristetraprolin (TTP) serves as a crucial prognostic biomarker for aggressive prostate cancer.
  • Augmenting TTP function presents a potential therapeutic strategy to inhibit prostate tumor metabolism and proliferation.
  • TTP plays a significant tumor-suppressive role in prostate cancer by impacting both gene expression and cellular metabolism.
Keywords:
TTPmetabolismproliferationprostate cancertristetraprolin

More Related Videos

An Orthotopic Murine Model of Human Prostate Cancer Metastasis06:48

An Orthotopic Murine Model of Human Prostate Cancer Metastasis

Published on: September 18, 2013

36.0K
A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer07:25

A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer

Published on: March 6, 2018

14.0K

Related Concept Videos

Drugs that Destabilize Microtubules01:10

Drugs that Destabilize Microtubules

Microtubules are dynamic structures and can be regulated by microtubule targeting agents (MTAs). Microtubule destabilizing drugs are a class of MTAs that destabilize and prevent microtubules' polymerization. Both natural and synthetic chemicals can be found under this class of drugs. Vincristine and vinblastine, two vinca alkaloids, and colchicine were among the first to be discovered. These drugs can affect cells in various ways, either by inducing a change in cell morphology, preventing...
4.2K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.4K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.0K
Drugs that Stabilize Microtubules01:15

Drugs that Stabilize Microtubules

Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...
2.8K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
6.1K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.7K