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Published on: August 16, 2019
Matrix metalloproteinase -9 polymorphism and outcome after acute myocardial infarction
Tarek A Abd El-Aziz1, Rasha H Mohamed2
1Cardiology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Insights
The study found that the MMP-9 TT genotype and elevated serum MMP-9 levels are linked to an increased risk of acute myocardial infarction (AMI) in Egyptian patients. These factors may also predict AMI outcomes.
Area of Science:
- Cardiovascular Genetics
- Molecular Cardiology
- Biomarker Discovery
Background:
- Matrix metalloproteinases (MMPs), particularly MMP-9, are implicated in the pathogenesis of coronary artery disease (CAD).
- Understanding the genetic and serum level associations of MMP-9 is crucial for assessing cardiovascular risk.
Purpose of the Study:
- To investigate the association between MMP-9 gene polymorphisms and serum levels with the risk of acute myocardial infarction (AMI) in an Egyptian cohort.
- To evaluate MMP-9 as a potential predictor of AMI outcomes within six months.
Main Methods:
- Genotyping of the MMP-9-1562C>T polymorphism was performed using PCR-based restriction digestion.
- Serum MMP-9 levels were quantified using ELISA assays.
- A cohort of 184 AMI patients and 180 controls were studied, with a six-month follow-up for complications.
Main Results:
- The MMP-9-1562T allele was more prevalent in AMI patients compared to controls (OR=1.65).
- The CT+TT genotypes were significantly associated with increased morbidity (OR=2.85) and mortality (OR=3.21) post-MI.
- Elevated serum MMP-9 levels correlated with AMI and the TT genotype, and were linked to impaired left ventricular function.
Conclusions:
- MMP-9 (TT genotype) and its serum levels are associated with AMI risk in Egyptians.
- MMP-9 polymorphism and serum levels show potential as clinical biomarkers for predicting AMI prognosis.
Objectives:
Matrix metalloproteinases (MMPs) play an important role in the pathogenesis of coronary artery disease (CAD). This study aims to determine the association of MMP-9 genotype polymorphisms and its serum levels with the risk of acute myocardial infarction (AMI) in Egyptian patients. Also, it evaluated their role as predictors of AMI outcome after six months follow-up.
Methods:
Subjects included in the study were 184 patients with AMI and 180 controls. Genotyping of MMP-9-1562C>T polymorphism was carried out by PCR-based restriction digestion method. Serum MMP-9 was measured using ELIZA assay. All patients were followed for AMI complications during their hospitalization and 6 months later on.
Results:
MMP-9-1562T allele was more frequent in patients than in controls (OR=1.65, 95%CI 1.09-2.15, P=0.011). the frequency of CT+TT genotypes were higher in patients with morbidity (OR=2.85, 95%CI 1.29-6.29, P=0.008) and with mortality (OR=3.21, 95%CI 1.28-8.02, P=0.012) than in those without MI complications. Serum MMP-9 levels were significantly elevated in AMI as compared to controls and more associated with TT genotype. The impairment of LV function (ΔEF, ΔLAD, ΔE/A) was more observed in the TT genotype compared with CC genotype.
Conclusions:
Our data suggest that MMP-9 (TT genotype) and its serum level are associated with the risk of suffering AMI in Egyptians. In addition, MMP-9 polymorphism and its level might be useful clinical biomarkers for predicting the outcome of AMI.
