Related Experiment Videos
S-100 protein in white preadipocytes: an immunoelectronmicroscopic study
S Cinti1, M Cigolini, M Morroni
1Istituto di Morfologia Umana Normale, Università di Ancona, Italy.
The Anatomical Record
|August 1, 1989
Summary
Identifying early preadipocytes is crucial for obesity research. This study found that S-100 protein is a reliable marker to distinguish early preadipocytes from fibroblasts, aiding in population assessment.
Area of Science:
- Cell Biology
- Histology
- Biochemistry
Background:
- Adipocyte differentiation is key to understanding obesity pathogenesis.
- Early preadipocytes lack distinct morphological features, making them indistinguishable from fibroblasts.
- Accurate identification of preadipocyte populations is currently impossible.
Purpose of the Study:
- To determine if S-100 protein can serve as a biochemical marker for early preadipocytes.
- To differentiate preadipocytes from fibroblasts at the ultrastructural level.
Main Methods:
- Utilized the peroxidase-antiperoxidase (PAP) preembedding method for S-100 immunoreactivity testing.
- Examined S-100 protein expression in preadipocytes and fibroblasts both in vivo and in cell culture.
- Analyzed ultrastructural morphology of cells.
Main Results:
- Mature adipocytes and early preadipocytes (including fibroblast-like cells) showed positive S-100 immunoreactivity.
- True fibroblasts, endothelial cells, and pericytes were negative for S-100 protein.
- Flattened, lipid-free, fibroblast-like cells surrounding pericytes were positive for S-100.
Conclusions:
- S-100 protein is a valuable biochemical marker for distinguishing early preadipocytes from fibroblasts.
- This finding facilitates accurate assessment of preadipocyte populations.
- Potential applications in obesity research and related fields.