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Draft Genomic Analysis of an Avian Multidrug Resistant Morganella morganii Isolate Carrying qnrD1
Daniela Jones-Dias1, Lurdes Clemente2, Inês B Moura1
1National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections, Department of Infectious Diseases, National Institute of Health Doutor Ricardo JorgeLisbon, Portugal; Centre for the Studies of Animal Science, Institute of Agrarian and Agri-Food Sciences and Technologies, Oporto UniversityOporto, Portugal.
Abstract:
Morganella morganii is a commensal bacterium and opportunistic pathogen often present in the gut of humans and animals. We report the 4.3 Mbp draft genome sequence of a M. morganii isolated in association with an Escherichia coli from broilers in Portugal that showed macroscopic lesions consistent with colisepticemia. The analysis of the genome matched the multidrug resistance phenotype and enabled the identification of several clinically important and potentially mobile acquired antibiotic resistance genes, including the plasmid-mediated quinolone resistance determinant qnrD1. Mobile genetic elements, prophages, and pathogenicity factors were also detected, improving our understanding toward this human and animal opportunistic pathogen.
Insights
This study sequenced the genome of Morganella morganii found in broiler chickens, identifying key antibiotic resistance genes like qnrD1. This improves understanding of this opportunistic pathogen in both humans and animals.
Area of Science:
- Microbiology
- Genomics
- Veterinary Medicine
Background:
- Morganella morganii is a common gut bacterium in humans and animals.
- It can act as an opportunistic pathogen, causing disease.
Purpose of the Study:
- To perform a draft genome sequence of a M. morganii strain isolated from broiler chickens in Portugal.
- To investigate the genetic basis of multidrug resistance and identify virulence factors.
Main Methods:
- Whole-genome sequencing of the M. morganii isolate.
- Bioinformatic analysis to identify genes, mobile genetic elements, and pathogenicity factors.
Main Results:
- A 4.3 Mbp draft genome sequence was obtained.
- The genome analysis confirmed the multidrug resistance phenotype.
- Identified clinically important antibiotic resistance genes, including the plasmid-mediated qnrD1.
- Detected mobile genetic elements, prophages, and pathogenicity factors.
Conclusions:
- The genome sequence provides insights into the genetic makeup of M. morganii.
- Understanding the genetic basis of resistance and virulence is crucial for managing this pathogen in both animal and human health.
- This study enhances knowledge of M. morganii as a significant opportunistic pathogen.
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