Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Human germline biallelic loss-of-function <i>OSMR</i> variants cause severe allergic disease.

Journal of human immunity·2026
Same author

Dual-Function Metal-Phenolic Network-Capped Starch Nanoparticles for Postharvest Pesticide Removal and Produce Preservation.

ACS nano·2026
Same author

Insulin receptor trafficking and interactions in muscle cells.

Journal of the Endocrine Society·2026
Same author

Normalized QRS Duration by LV Dimension: Eliminating Sex-Specific Differences and Predicting Cardiac Resynchronization Therapy Outcomes.

JACC. Clinical electrophysiology·2026
Same author

Acute Thrombosis During Atrial Leadless Pacemaker Implantation.

JACC. Case reports·2025
Same author

The tropomyosin 3.1/3.2 inhibitor ATM-3507 alters B-cell actin dynamics and impairs the growth and motility of diffuse large B-cell lymphoma cell lines.

Frontiers in immunology·2025

Related Experiment Video

Updated: Mar 12, 2026

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
06:25

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

8.9K

Real-time 3D stabilization of a super-resolution microscope using an electrically tunable lens.

Reza Tafteh, Libin Abraham, Denny Seo

    Optics Express
    |November 10, 2016
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces an electrically tunable lens (ETL) system to stabilize single-molecule localization microscopy (SMLM) and prevent drift artifacts. The new system enables more accurate analysis of biological structures like transferrin receptors in B-lymphocytes.

    More Related Videos

    Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging
    10:01

    Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

    Published on: September 8, 2017

    8.3K
    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
    08:41

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

    Published on: August 16, 2012

    12.1K

    Related Experiment Videos

    Last Updated: Mar 12, 2026

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
    06:25

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

    Published on: February 12, 2014

    8.9K
    Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging
    10:01

    Demonstration of a Hyperlens-integrated Microscope and Super-resolution Imaging

    Published on: September 8, 2017

    8.3K
    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
    08:41

    Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

    Published on: August 16, 2012

    12.1K

    Area of Science:

    • Biophysics
    • Microscopy
    • Cell Biology

    Background:

    • Single-molecule localization microscopy (SMLM) is crucial for visualizing biological structures.
    • Long acquisition times in SMLM lead to drift-induced artifacts, limiting image accuracy.
    • Accurate imaging is essential for understanding cellular processes and molecular organization.

    Purpose of the Study:

    • To develop and validate an active optical stabilization system for SMLM.
    • To mitigate mechanical drift artifacts during 3D SMLM imaging.
    • To improve the accuracy of analyzing molecular cluster topology in biological samples.

    Main Methods:

    • Implementation of an optical design featuring an electrically tunable lens (ETL).
    • Utilizing a bifocal design with fiducial markers on the coverslip for sample stabilization.
    • Testing the system by imaging endosomal transferrin receptors in B-lymphocytes at an 8 µm depth.

    Main Results:

    • The ETL system achieved near-elimination of mechanical drift (RMS ~0.7 nm lateral, ~2.7 nm axial).
    • Stabilization was effective across different imaging depths.
    • Drift-free imaging revealed distinct, heterogeneous clusters of transferrin receptors with a bimodal size distribution, unlike unimodal distributions without stabilization.

    Conclusions:

    • The ETL-based stabilization system significantly enhances the accuracy of SMLM imaging.
    • It enables more precise analysis of molecular cluster topology, as demonstrated with transferrin receptors.
    • The system is cost-effective and compatible with existing microscopy setups.