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Permeability and penicillin-binding protein alterations in Salmonella muenchen: stepwise resistance acquired during
F Bellido1, I R Vladoïanu, R Auckenthaler
1Département de Microbiologie, Centre Médical Universitaire de Genève, Switzerland.
Abstract:
A patient with Salmonella muenchen sepsis was unsuccessfully treated with ampicillin. During therapy, four strains that showed stepwise ampicillin resistance and affected other beta-lactams and unrelated antibiotics were isolated sequentially. Resistance was caused by decreased outer membrane permeability associated with diminished expression of porin OmpF. Furthermore, the most resistant isolate overproduced the PBP 3 target molecule.
Insights
Salmonella sepsis developed ampicillin resistance during treatment. This resistance stemmed from reduced porin OmpF expression and altered PBP 3, impacting multiple antibiotics.
Area of Science:
- Microbiology
- Infectious Diseases
- Molecular Biology
Background:
- Salmonella muenchen sepsis is a serious infection.
- Ampicillin is a common antibiotic treatment for bacterial infections.
Observation:
- A patient with Salmonella sepsis showed resistance to ampicillin during treatment.
- Sequential isolation of four resistant Salmonella strains occurred during therapy.
Findings:
- The Salmonella strains developed stepwise resistance to ampicillin and other beta-lactams.
- Resistance was linked to decreased outer membrane permeability due to diminished porin OmpF expression.
- The most resistant isolate also overproduced the penicillin-binding protein 3 (PBP 3).
Implications:
- This case highlights the rapid development of multidrug resistance in Salmonella during antibiotic therapy.
- Understanding these resistance mechanisms is crucial for effective sepsis treatment.
- Alterations in porin expression and target molecule production can lead to treatment failure.