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Novel amperometric glucose biosensor based on MXene nanocomposite.

R B Rakhi1,2, Pranati Nayak1, Chuan Xia1

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This study introduces a novel Au/MXene nanocomposite biosensor for highly sensitive glucose detection. This advanced electrochemical biosensor demonstrates excellent performance, paving the way for improved diagnostic tools.

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Area of Science:

  • Electrochemistry
  • Materials Science
  • Biotechnology

Background:

  • Development of sensitive and stable biosensors is crucial for accurate disease diagnosis.
  • Nanocomposite materials offer unique properties for enhanced biosensing applications.
  • MXene and gold nanoparticles (Au) exhibit synergistic effects beneficial for electrochemical detection.

Purpose of the Study:

  • To develop a novel amperometric glucose biosensor using an Au/MXene nanocomposite.
  • To investigate the electrocatalytic properties of the Au/MXene nanocomposite for glucose oxidase (GOx) immobilization.
  • To evaluate the analytical performance, stability, and reproducibility of the fabricated biosensor.

Main Methods:

  • Fabrication of a glassy carbon electrode (GCE) modified with Nafion-solubilized Au/MXene nanocomposite.
  • Immobilization of glucose oxidase (GOx) enzyme onto the modified electrode.
  • Amperometric detection of glucose concentration using cyclic voltammetry and chronoamperometry.
  • Characterization of the biosensor's sensitivity, detection limit, stability, and reproducibility.

Main Results:

  • The GOx/Au/MXene/Nafion/GCE biosensor showed a linear response to glucose in the range of 0.1–18 mM.
  • A high sensitivity of 4.2 μA mM⁻¹ cm⁻² was achieved.
  • A low detection limit of 5.9 μM (S/N = 3) was obtained, indicating high sensitivity.
  • The biosensor demonstrated excellent stability, reproducibility, and repeatability.

Conclusions:

  • The Au/MXene nanocomposite serves as an effective electrochemical transducer for sensitive enzymatic glucose detection.
  • The synergistic interaction between Au nanoparticles and MXene sheets enhances the biosensor's performance.
  • This developed biosensor platform holds significant potential for applications in electrochemical biosensing and diagnostics.