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Image analysis for a microfluidic paper-based analytical device using the CIE L*a*b* color system.

Takeshi Komatsu1, Saeed Mohammadi1, Lori Shayne Alamo Busa1

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Summary

This study presents a new method for quantitative analysis using microfluidic paper-based analytical devices (μPADs) and digital image analysis. The approach utilizes multiple color changes for accurate pH measurements across a broad range.

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Area of Science:

  • Analytical Chemistry
  • Materials Science

Background:

  • Microfluidic paper-based analytical devices (μPADs) are valuable tools for simple and accessible quantitative analysis.
  • Digital image analysis enhances the precision and scope of μPAD applications.
  • Traditional analysis methods for μPADs often rely on grayscale, limiting their dynamic range.

Purpose of the Study:

  • To demonstrate a novel quantitative analysis method for μPADs using multiple color changes.
  • To expand the detectable pH range for μPAD-based measurements.
  • To evaluate the effectiveness of the CIE L*a*b* color system for μPAD image analysis.

Main Methods:

  • Utilized microfluidic paper-based analytical devices (μPADs) for sample analysis.
  • Employed digital image analysis with the CIE L*a*b* color system to interpret colorimetric data.
  • Used a universal pH indicator capable of multiple color changes across a spectrum of pH values.

Main Results:

  • Successfully performed quantitative pH measurements using multiple color changes on μPADs.
  • Achieved a wider detectable pH range compared to traditional grayscale analysis methods.
  • Demonstrated accurate pH measurements for aqueous solutions in the range of 2-9.

Conclusions:

  • The integration of multiple color analysis with the CIE L*a*b* system significantly enhances the quantitative capabilities of μPADs.
  • This method offers a broader and more sensitive approach for pH determination using paper-based devices.
  • The developed technique provides a robust platform for various analytical applications requiring wide-range quantitative measurements.