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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Mar 12, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

Review: imaging technologies for flow cytometry.

Yuanyuan Han¹, Yi Gu¹, Alex Ce Zhang¹

¹Department of Electrical and Computer Engineering, University of California, San Diego, California 92093, USA. ylo@ucsd.edu.

|November 11, 2016

Summary

This summary is machine-generated.

High-throughput single cell imaging, using advanced imaging flow cytometry, is revolutionizing biological and medical research. This technology merges microscopy

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Last Updated: Mar 12, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry

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Published on: January 7, 2019

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Published on: August 15, 2013

Area of Science:

Molecular and Cellular Biology
Biotechnology
Medical Research

Background:

High-throughput single cell imaging is a crucial technology driving advancements across multiple scientific disciplines.
Imaging flow cytometry integrates microscopy's imaging power with flow cytometry's high-throughput analysis capabilities.

Purpose of the Study:

To describe recent technological advancements in imaging flow cytometry.
To highlight the challenges associated with these new imaging flow cytometry technologies.

Main Methods:

Review of recent developments in imaging flow cytometry instrumentation and techniques.
Analysis of the integration of imaging and high-throughput analysis for single cells.

Main Results:

Recent innovations are significantly enhancing the capabilities of single-cell analysis.
Imaging flow cytometry is transforming the landscape of cellular research.

Conclusions:

Imaging flow cytometry represents a major leap forward in single-cell analysis.
Continued development and addressing current challenges will further expand its applications in biology and medicine.