Related Experiment Video
Updated: Mar 12, 2026

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
Published on: April 25, 2025
CRISPR-cas loci profiling of Cronobacter sakazakii pathovars
Pauline Ogrodzki1, Stephen James Forsythe1
1Pathogen Research Group, School of Science & Technology, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, UK.
Aim:
Cronobacter sakazakii sequence types 1, 4, 8 and 12 are associated with outbreaks of neonatal meningitis and necrotizing enterocolitis infections. However clonality results in strains which are indistinguishable using conventional methods. This study investigated the use of clustered regularly interspaced short palindromic repeats (CRISPR)-cas loci profiling for epidemiological investigations.
Materials & Methods:
Seventy whole genomes of C. sakazakii strains from four clonal complexes which were widely distributed temporally, geographically and origin of source were profiled.
Results & Conclusion:
All strains encoded the same type I-E subtype CRISPR-cas system with a total of 12 different CRISPR spacer arrays. This study demonstrated the greater discriminatory power of CRISPR spacer array profiling compared with multilocus sequence typing, which will be of use in source attribution during Cronobacter outbreak investigations.
More Related Videos
Related Concept Videos
CRISPR and crRNAs
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
CRISPR
CRISPR
The Antiviral System of Bacteria and Archaea: CRISPR
CRISPR/Cas9 Genome Editing

