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The roles of factors from lung in murine megakaryocytopoiesis
J Fawcett1, O S Huat, N Williams
1Department of Physiology, University of Melbourne, Parkville, Victoria, Australia.
Abstract:
The roles of factors from mouse lung in stimulating murine megakaryocytopoiesis were examined. Conditioned medium from normal mice was found to contain interleukin 3 (IL-3) activity in addition to granulocyte-macrophage colony-stimulating factor (GM-CSF) and megakaryocyte potentiator (Mk-potentiator). The Mk-potentiator activity of mouse lung-conditioned medium (MLCM) was found to be immunologically distinct from IL-3. Biochemical separation of MLCM showed Mk-potentiator activity with an activity profile distinct from IL-3 and GM-CSF. When titrated, Mk-potentiator was the major activity enhancing megakaryocyte colony formation in MLCM. By contrast, at high concentrations of MLCM, all factors were present and may play a role in megakaryocyte colony growth and development.