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Determination of Alternate Splicing Events Using Transcriptome Arrays.
Rebecca S DeVaux1, Marcy Kuentzel2, Jason Herschkowitz2
1Department of Biomedical Sciences, School of Public Health, Cancer Research Center, Rensselaer, NY, 12144, USA.
Methods in Molecular Biology (Clifton, N.J.)
|November 11, 2016
Summary
Differential isoform expression analysis is crucial for understanding biology and disease. The Affymetrix GeneChip Human Transcriptome Array 2.0 enables comprehensive examination of all known transcript isoforms.
Area of Science:
- Molecular Biology
- Genomics
- Transcriptomics
Background:
- Understanding differential isoform expression is key to elucidating biological mechanisms in development and disease.
- Current high-throughput expression profiling of splice variants faces limitations in sample requirements and analytical platforms.
Purpose of the Study:
- To introduce and describe the Affymetrix GeneChip Human Transcriptome Array 2.0.
- To provide a platform for comprehensive examination of all known human transcript isoforms.
Main Methods:
- Utilized the Affymetrix GeneChip Human Transcriptome Array 2.0.
- Performed high-throughput expression profiling analysis.
Main Results:
- The GeneChip Human Transcriptome Array 2.0 allows for comprehensive examination of all known transcript isoforms.
- This platform addresses limitations of previous methods in quantitation and analysis.
Conclusions:
- The Affymetrix GeneChip Human Transcriptome Array 2.0 is a valuable tool for studying differential isoform expression.
- This technology facilitates a deeper understanding of the biological basis of development and disease states.
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