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Rrp15 affects cell cycle, proliferation, and apoptosis in NIH3T3 cells
Tao Wu1, Mei-Xia Ren1, Guo-Ping Chen2
1Department of Cardiology The First Affiliated Hospital School of Medicine Zhejiang University Hangzhou China.
FEBS Open Bio
|November 12, 2016
Summary
The Rrp15 gene knockdown suppressed cell proliferation and induced apoptosis in NIH3T3 cells. Rrp15 protein, found in the nucleus, binds RNA and affects proteasome activity, promoting cell growth.
Area of Science:
- Molecular Biology
- Cell Biology
- Genetics
Background:
- Riken 2810430M08 (Rrp15) is a newly identified mouse gene.
- Rrp15 protein is homologous to yeast RRP15, involved in ribosomal RNA processing.
- Previous work suggested a link between Rrp15 and T cell proliferation/activation.
Purpose of the Study:
- To investigate the function of Rrp15 in apoptosis and cell proliferation.
- To determine Rrp15's role in RNA binding within the nucleus.
- To explore Rrp15's impact on 20S proteasome activity.
Main Methods:
- Constructed Rrp15 gene knockdown in NIH3T3 cells.
- Utilized real-time PCR, western blotting, flow cytometry, and immunofluorescence.
- Analyzed Rrp15 protein localization and RNA binding in the nucleus.
Main Results:
- Rrp15 knockdown suppressed NIH3T3 cell proliferation and induced apoptosis.
- Rrp15 protein localizes to the nucleus and binds RNA/pre-RNA.
- Rrp15 altered 20S proteasome activity.
Conclusions:
- Rrp15 promotes proliferation and inhibits apoptosis in NIH3T3 cells.
- Rrp15 is involved in nuclear RNA processing or regulation.
- Rrp15 may play a significant role in cellular growth and survival pathways.
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