Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Experimental synovitis induced by collagen-specific T cell lines.

E Brahn1, D E Trentham

  • 1Department of Medicine, UCLA School of Medicine 90024.

Cellular Immunology
|February 1, 1989
PubMed
Summary

Type II collagen-reactive T cells can cause sustained synovitis in rats, independent of antibodies. This experimental arthritis is linked to an arthritogenic lymphokine release.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Troponin I Assessment of Cardiac Involvement in Patients With Connective Tissue Disease and an Elevated Creatine Kinase MB Isoform Report of Four Cases and Review of the Literature.

Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases·2008
Same author

Amiprilose hydrochloride for the treatment of rheumatoid arthritis.

Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases·2008
Same author

Treatment of rheumatic disease by tumor necrosis factor-alpha blockade: knowns and unknowns.

Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases·2006
Same author

Environmental modulation of autoimmune arthritis involves the spontaneous microbial induction of T cell responses to regulatory determinants within heat shock protein 65.

Journal of immunology (Baltimore, Md. : 1950)·2001
Same author

Taxol (paclitaxel) involution of articular cartilage destruction in collagen induced arthritis: an ultrastructural demonstration of an increased superficial chondroprotective layer.

The Journal of rheumatology·2000
Same author

Suppression of coronary vasculitis in a murine model of Kawasaki disease using an angiogenesis inhibitor.

Clinical immunology (Orlando, Fla.)·1999

Area of Science:

  • Immunology
  • Rheumatology
  • Cell Biology

Background:

  • Type II collagen (CII) is a major component of cartilage.
  • Ovalbumin (OV) serves as a control antigen.
  • T cell lines are crucial for studying immune responses.

Purpose of the Study:

  • To investigate the role of T cells in collagen-induced arthritis.
  • To identify the mechanisms underlying T cell-mediated synovitis.
  • To explore the involvement of lymphokines in arthritis development.

Main Methods:

  • Developing antigen-specific T cell lines from immunized rats.
  • Intra-articular injection of T cell lines into rat knees.
  • Histological analysis of joint tissues to assess synovitis and pannus formation.
  • Evaluating the role of antibodies and lymphokines in the disease process.

Main Results:

  • Type II collagen (CII) reactive T cell lines induced significant synovitis and pannus formation.
  • The induced arthritis required viable lymphocytes and was dose-dependent.
  • Arthritis development was independent of anticollagen antibodies.
  • A 65-kDa CII-binding arthritogenic lymphokine was associated with synovitis-inducing T cell lines.
  • Intravenous administration of CII cells followed by intra-articular challenge also induced synovitis.

Conclusions:

  • T helper cell lines specific for Type II collagen can induce a sustained, antibody-independent synovitis.
  • The experimental arthritis model is associated with the release of an arthritogenic lymphokine.
  • These findings highlight the role of T cells and lymphokines in the pathogenesis of autoimmune arthritis.

Related Experiment Videos