Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Control of IgE responses.

H G Durkin1, D L Auci, S M Chice

  • 1Department of Pathology, State University of New York Health Science Center, Brooklyn 11203.

Clinical Immunology and Immunopathology
|January 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chlamydia pneumoniae induces interleukin-12 responses in peripheral blood mononuclear cells in asthma and the role of toll like receptor 2 versus 4: a pilot study.

Irish journal of medical science·2016
Same author

Increased seroprevalence of Enterovirus 71 IgE antibodies in asthmatic compared with non-asthmatic children.

Irish journal of medical science·2016
Same author

Anticorps Monoclonaux: Principes Fondamentaux Et Applications.

Acta clinica Belgica·2016
Same author

The lymphocytes of splenic marginal zones: a distinct B-cell lineage.

Immunology today·2014
Same author

More on rat monoclonal antibodies.

Immunology today·2014
Same author

Allergic disease is associated with epilepsy in childhood: a US population-based study.

Allergy·2013
Same journal

Mechanisms of virus replication, persistence, and regulation of immune responses.

Clinical immunology and immunopathology·2020
Same journal

Cumulative subject index for volumes 86-89

Clinical immunology and immunopathology·1998
Same journal

D-penicillamine-induced pancreatic islet autoantibody production is independent of the immunogenetic background: a lesson from patients with Wilson's disease.

Clinical immunology and immunopathology·1998
Same journal

Characterization of gastric Na+/I- symporter of the rat.

Clinical immunology and immunopathology·1998
Same journal

CD8(+), radiosensitive T cells of parental origin, oppose cells capable of down-regulating cytotoxicity in murine acute lethal graft-versus-host disease.

Clinical immunology and immunopathology·1998
Same journal

Specific activity of alpha1proteinase inhibitor and alpha2macroglobulin in human serum: application to insulin-dependent diabetes mellitus.

Clinical immunology and immunopathology·1998
See all related articles

Germ-free rats and hyper-IgE syndrome patients show altered Peyer's patches with increased IgE-bearing B cells and specific T cell subsets. Restoration occurred with microbial components or thymectomy, highlighting the role of gut microbiota in immune development.

Area of Science:

  • Immunology
  • Gastroenterology
  • Microbiology

Background:

  • Peyer's patches (PP) are crucial for intestinal immunity.
  • Germ-free (GF) animals and individuals with hyper-IgE syndrome (HIES) exhibit distinct immune profiles.
  • The role of gut microbiota and thymic education in PP development and cellular composition is not fully understood.

Purpose of the Study:

  • To investigate the cellular composition of Peyer's patches in germ-free rats and hyper-IgE syndrome patients.
  • To determine the impact of microbial components and thymectomy on Peyer's patch cellularity and immune cell subsets.
  • To explore the origin and localization of IgE-bearing B cells in the context of gut immunity.

Main Methods:

  • Comparative analysis of Peyer's patches from germ-free rats, conventional rats, HIES patients, and healthy humans.

Related Experiment Videos

  • Flow cytometry to identify B and T cell subsets (sIgE+, sIgA+, sIgM+, T cell phenotypes).
  • Intervention studies involving standard chow, bacterial cell wall components, and thymectomy in GF rats.

    • Main Results:

    • GF rats and HIES patients had fewer Peyer's patches with altered cellularity and distinct B/T cell populations, including high numbers of IgE-bearing B cells and suppressor T cells.
    • Administration of standard chow, bacterial components, or thymectomy normalized Peyer's patch cellularity, eliminated IgE+ B cells, and restored T cell balance in GF rats.
    • IgE+ B cells in HIES patients were primarily found in mesenteric lymph nodes, not in lymphoid follicles, suggesting an aberrant immune response.

    Conclusions:

    • Gut microbiota and thymic influence are critical for the normal development and immune cell composition of Peyer's patches.
    • The presence of IgE-bearing B cells and specific T cell subsets in GF rats and HIES patients indicates a dysregulated immune system.
    • Restoration of normal Peyer's patch architecture and immune cell populations can be achieved through microbial stimulation or thymic education.