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Metabolic Engineering for Probiotics and their Genome-Wide Expression Profiling
Ruby Yadav1, Puneet K Singh1, Pratyoosh Shukla1
1Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak-124001, Haryana, India.
Current Protein & Peptide Science
|November 12, 2016
Summary
Metabolic engineering enhances understanding and design of probiotic bacteria for the food industry. These methods improve functions like exopolysaccharide production and explore metabolic pathways for better strain performance.
Area of Science:
- Microbiology and Biotechnology
- Food Science and Technology
Background:
- Probiotic supplements are increasingly important in the food industry.
- Metabolic engineering (ME) is key to understanding and designing probiotic functions.
- Lactic acid bacteria (LAB) are crucial industrial probiotics for fermentation.
Purpose of the Study:
- To review metabolic engineering approaches for industrial probiotic bacteria.
- To explore methods for understanding probiotic mechanisms and improving strain performance.
- To discuss the integration of ME with genome profiling for advanced probiogenomics.
Main Methods:
- Transcriptomic studies (e.g., Lactobacillus reuteri).
- Genetic modification for enhanced biosynthesis (e.g., Lactobacillus casei LC2W exopolysaccharide yield).
- Systems biology tools, including in silico design and genome profiling.
Main Results:
- Demonstrated improvements in exopolysaccharide (EPS) biosynthesis.
- Enabled exploration of metabolic pathways and cellular metabolic states.
- Facilitated effective genome editing and introduction of novel enzymes.
Conclusions:
- Metabolic engineering offers powerful tools for optimizing probiotic bacteria.
- Integration of ME with genome profiling advances understanding of metabolic interactions and fluxomics.
- Future research can leverage these approaches for enhanced probiotic applications in Bifidobacterium and Lactobacillus species.
