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Wide-Spectrum Microscope with a Long Working Distance Aspherical Objective Based on Obscuration Constraint.

Weibo Wang1,2, Chao Wang3, Jian Liu4

  • 1Institute of Ultra-Precision Optoelectronic Instrument Engineering, Harbin Institute of Technology, Harbin 150001, China. weibo.wang@eng.ox.ac.uk.

Sensors (Basel, Switzerland)
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Summary

This study introduces a novel design method for a long working distance microscope using an aspherical Schwarzschild objective. The approach enables wide-spectrum imaging with a low obscuration, validated through resolution testing.

Keywords:
aspherical Schwarzschild objectivelong working distancelow obscurationwide-spectrum microscope

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Area of Science:

  • Optical Engineering
  • Microscopy Design

Background:

  • Designing long working distance microscopes with low obscuration is challenging.
  • Aspherical Schwarzschild objectives offer potential for improved optical performance.

Purpose of the Study:

  • To present a new design approach for a long working distance microscope.
  • To achieve wide-spectrum imaging with a low obscuration aspherical Schwarzschild objective.

Main Methods:

  • Utilized obscuration constraint and on-axis Taylor series expansion for initial configuration design.
  • Designed a microscope with numerical aperture (NA) = 0.13 and working distance (WD) = 525 mm.
  • Employed wide-spectrum imaging (λ = 400-900 nm).

Main Results:

  • Successfully designed a low obscuration aspherical Schwarzschild objective.
  • Verified the design's validity through experiments on resolution targets and USAF charts.
  • Tested performance with various wavelength light sources (480-850 nm) and a line charge-coupled device (CCD).

Conclusions:

  • The proposed design approach is effective for realizing long working distance microscopes.
  • The developed objective enables efficient wide-spectrum imaging.
  • Experimental validation confirms the method's practical applicability.