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Related Concept Videos

Spermatogenesis01:41

Spermatogenesis

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Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
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Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...
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Related Experiment Video

Updated: Mar 12, 2026

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye
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Transcriptomic Variation during Spermiogenesis in Mouse Germ Cells.

Haiyang Zuo1,2, Junfang Zhang1, Liuguang Zhang1,3

  • 1The Key Laboratory for Farm Animal Genetic and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing, 100193, China.

Plos One
|November 12, 2016
PubMed
Summary
This summary is machine-generated.

This study reveals significant changes in gene transcription and alternative splicing during mouse spermiogenesis. Researchers identified novel gene expressions and differentially expressed genes crucial for sperm development.

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Area of Science:

  • Reproductive Biology
  • Genomics
  • Molecular Biology

Background:

  • Spermiogenesis involves complex transcriptional regulation.
  • Understanding gene expression dynamics is key to male fertility.

Purpose of the Study:

  • To investigate transcriptional variations during mouse spermiogenesis.
  • To identify novel transcripts and alternative splicing events.

Main Methods:

  • Laser capture microdissection (LCM) of round and elongated spermatids.
  • RNA sequencing (RNA-seq) of transcripts from spermatids and sperm.
  • Bioinformatic analysis including read mapping and gene expression profiling.

Main Results:

  • Sperm showed significantly lower read mapping to known exons (9%) compared to spermatids (70%).
  • Complex alternative splicing patterns were observed in sperm.
  • 5,104 de novo genes and 91,112 alternative splicing events were detected.
  • 12,105 differentially expressed genes (DEGs) were identified.

Conclusions:

  • Spermiogenesis involves extensive transcriptomic remodeling, including novel gene expression and alternative splicing.
  • Changes in gene transcription impact mitochondrial function and energy metabolism during sperm development.
  • This study provides a comprehensive resource for understanding mouse spermiogenesis transcriptomics.