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Updated: Mar 12, 2026

Investigation into Deep Breathing through Measurement of Ventilatory Parameters and Observation of Breathing Patterns
Published on: September 16, 2019
Improving respiration measurements with gas exchange analyzers
R Montero1, M Ribas-Carbó2, N F Del Saz2
1Institut de Recerca i Formació Agrària i Pesquera, Conselleria d'Agricultura, Medi Ambient i Territori. Govern de les Illes Balears, C/Eusebio Estada n° 145, 07009 Palma de Mallorca, Spain.
Abstract:
Dark respiration measurements with open-flow gas exchange analyzers are often questioned for their low accuracy as their low values often reach the precision limit of the instrument. Respiration was measured in five species, two hypostomatous (Vitis Vinifera L. and Acanthus mollis) and three amphistomatous, one with similar amount of stomata in both sides (Eucalyptus citriodora) and two with different stomata density (Brassica oleracea and Vicia faba). CO2 differential (ΔCO2) increased two-fold with no change in apparent Rd, when the two leaves with higher stomatal density faced outside. These results showed a clear effect of the position of stomata on ΔCO2. Therefore, it can be concluded that leaf position is important to guarantee the improvement of respiration measurements increasing ΔCO2 without affecting the respiration results by leaf or mass units. This method will help to increase the accuracy of leaf respiration measurements using gas exchange analyzers.
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