Related Experiment Videos
Cell surface endothelial proteins altered in experimental allergic encephalomyelitis.
N H Sternberger1, L A Sternberger, M W Kies
1Department of Anatomy, University of Maryland School of Medicine, Baltimore 21201.
Journal of Neuroimmunology
|February 1, 1989
Summary
Researchers identified changes in blood-brain barrier (BBB) endothelial cells during experimental allergic encephalomyelitis (EAE). The anti-endothelial barrier antigen (EBA) antibody revealed altered expression in EAE lesions and during recovery, offering insights into BBB breakdown.
Area of Science:
- Neuroimmunology
- Vascular Biology
- Cell Biology
Background:
- Endothelial cells (EC) play a role in inflammatory and immune responses.
- Experimental allergic encephalomyelitis (EAE) is a model for studying inflammatory diseases affecting the central nervous system.
Purpose of the Study:
- To investigate alterations in central nervous system endothelium during EAE.
- To explore the utility of anti-endothelial barrier antigen (EBA) antibody in studying blood-brain barrier (BBB) changes.
Main Methods:
- Utilized a mouse monoclonal antibody (anti-EBA) targeting luminal plasma membrane of CNS endothelium.
- Examined microvessels in normal, EAE-affected, and recovered Lewis rats' brains and spinal cords.
Main Results:
- Anti-EBA reacted with microvessels in normal rat CNS but showed abolished reaction in EAE lesions with inflammatory cells.
- EBA was reexpressed in most ECs during recovery from EAE, but blood vessels in residual lesion areas remained negative.
- Identified alterations in BBB associated with EAE lesions.
Conclusions:
- Anti-EBA antibody is a valuable tool for detecting BBB alterations in EAE.
- EBA expression changes during EAE pathogenesis and recovery.
- Further research is needed to understand the biochemical changes and mediators affecting EBA binding in EAE.