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Cementum structure in Beluga whale teeth.

S R Stock1, L A Finney2, A Telser1

  • 1Dept. of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.

Acta Biomaterialia
|November 13, 2016
PubMed
Summary

Beluga whale teeth cementum growth bands (GLGs) show mineral content variations, not collagen differences. Zinc intensity is a sensitive indicator of biomineralization changes in these whale age markers.

Keywords:
CaCementumDentinOdontoceteSynchrotron radiationX-ray diffractionX-ray fluorescenceZngrowth layer groups (GLGs)

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Area of Science:

  • Paleontology
  • Biomineralization
  • Zoology

Background:

  • Beluga whale (Delphinapterus leucas) teeth cementum contains annular growth bands (GLGs) crucial for age determination.
  • Cementum, a mineralized tissue, attaches periodontal ligaments and is primarily carbonated apatite and collagen.
  • Previous studies suggested collagen orientation differences cause GLG banding, not mineral content variations.

Purpose of the Study:

  • To investigate the microstructural composition of Beluga whale cementum growth layer groups (GLGs).
  • To determine the factors responsible for the formation of annual light-dark bands in Beluga whale cementum.
  • To assess the role of zinc in the biomineralization process within cementum.

Main Methods:

  • Synchrotron X-ray fluorescence mapping to analyze elemental distribution (Ca, Zn).
  • Synchrotron X-ray diffraction mapping to analyze mineral phase (carbonated hydroxyapatite - cAp).
  • Microbeam analysis of Beluga whale teeth cementum thin sections.

Main Results:

  • Calcium (Ca) and Zinc (Zn) fluorescent intensities correlate with carbonated hydroxyapatite (cAp) diffracted intensities, matching visible light-dark bands.
  • The ratio of Zn to Ca is slightly higher in bands with maximum Ca and Zn intensity.
  • GLG modulation of cAp, Ca, and Zn is preserved for over 25 years in the cementum.
  • Banding in Beluga whale cementum is attributed to differences in mineral content, not collagen orientation.

Conclusions:

  • Beluga whale cementum growth bands result from variations in mineral content, specifically Ca and Zn concentrations.
  • Zinc plays a significant role in cementum biomineralization, and its fluorescent intensity is a sensitive indicator of these changes.
  • The findings clarify the microstructural basis of GLGs in Beluga whale teeth, supporting their use in age estimation.