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Updated: Mar 12, 2026

Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions

Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions

Published on: October 13, 2015

Fractionation Techniques to Examine Effector Translocation.

Rachel M Olson¹, Deborah M Anderson²

¹Department of Veterinary Pathobiology, University of Missouri-Columbia, E111 Veterinary Medicine Bldg, Columbia, MO, 65211, USA.

Methods in Molecular Biology (Clifton, N.J.)

|November 13, 2016

Summary

This summary is machine-generated.

This study details methods for analyzing bacterial effector proteins translocated into host cells via type III secretion systems (T3SS). Understanding these protein interactions is crucial for studying bacterial pathogenesis and host cell manipulation.

Keywords:

Digitonin Fractionation Subcellular fractionation Translocation

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Measuring Peptide Translocation into Large Unilamellar Vesicles

Measuring Peptide Translocation into Large Unilamellar Vesicles

Published on: January 27, 2012

Area of Science:

Microbiology
Cell Biology
Pathogenesis

Background:

Gram-negative bacterial pathogens utilize type III secretion systems (T3SS) to deliver effector proteins into host cells.
These effectors manipulate host cell signaling, trafficking, and intracellular processes, crucial for bacterial survival and pathogenesis.
Both extracellular and intracellular bacteria employ T3SS for host cell invasion and modulation.

Purpose of the Study:

To provide detailed methods for analyzing translocated effector proteins.
To enable the study of protein-protein interactions and host cell impacts of T3SS effectors.
To facilitate the characterization of mutant strains affecting effector translocation.

Main Methods:

Biochemical fractionation of infected host cells in vitro.
Mechanical fractionation procedures to isolate cellular components.
Analysis of translocated effector proteins and their interactions.

Main Results:

Established protocols for detecting and analyzing T3SS translocated effector proteins.
Enabled identification of protein-protein interactions mediated by effectors.
Facilitated characterization of effector protein functions and host cell perturbations.

Conclusions:

Biochemical and mechanical fractionation are effective methods for studying T3SS effector translocation.
These methods are essential for understanding bacterial pathogenesis mechanisms.
The provided techniques aid in identifying therapeutic targets against bacterial infections.