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Updated: Mar 12, 2026

Quantifying Yersinia pseudotuberculosis Type III Secretion System Activity Following Iron Starvation and Anaerobic Growth
Published on: May 31, 2024
A Method for Characterizing the Type III Secretion System's Contribution to Pathogenesis: Homologous Recombination to
Patrick Osei-Owusu1, Matthew L Nilles2, David S Bradley3
1Department of Microbiology, University of Chicago, Chicago, IL, USA.
Abstract:
The type III (T3S) secretion system of many gram-negative bacteria is a surface-exposed protein secretion apparatus used to directly inject bacterial effector molecules into eukaryotic cells. These effector molecules contribute to bacterial pathogenesis in many ways, and have been shown to be crucial for infectivity. Here, we describe a protocol for using homologous recombination to generate T3S system mutants to assess the role of different T3S system proteins in bacterial pathogenesis.
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